国兰叶色突变体叶片差异表达基因分析

doi:10.16420/j.issn.0513-353x.2021-0950

摘要/Abstract

摘要：

中国兰春剑‘隆昌素’（Cymbidium longibracteaturn）及其叶色突变体‘叶艺隆昌素’是研究兰花叶色突变形成机制的良好材料。采用Illumina Hiseq2500对两种材料的叶片进行de novo转录组测序，以此鉴定与叶色突变性状形成相关的差异表达基因。结果表明，色素合成、叶绿体发育相关基因可能是导致‘隆昌素’与‘叶艺隆昌素’叶片性状差异的原因。

关键词: 国兰, 叶片, 叶色, 转录组

Abstract:

The Chinses orchid‘Longchangsu’and its leaf color mutant‘Yeyi Longchangsu’are excellent materials to study the formation mechanism of the leaf color of orchid. Differentially expressed genes（DEGs）in the leaves of‘Longchangsu’and‘Yeyi Longchangsu’were identifed by next-generation sequencing using Illumina Hiseq2500. Through the analysis of different expression genes，it was found that the genes related to pigment synthesis and chloroplast development might be the cause of the differences between‘Longchangsu’and‘Yeyi Longchangsu’.

Key words: Chinese orchid, leaf, leaf color, transcriptome

中图分类号:

S682.31

蒋彧, 涂勋良, 何俊蓉. 国兰叶色突变体叶片差异表达基因分析[J]. 园艺学报, 2023, 50(2): 371-381.

JIANG Yu, TU Xunliang, HE Junrong. Analysis of Differential Expression Genes in Leaves of Leaf Color Mutant of Chinese Orchid[J]. Acta Horticulturae Sinica, 2023, 50(2): 371-381.

图/表 10

参考文献 35

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基因名称 Gene name	引物序列（5′-3′） Primers sequence
c19370_g1	F：CGCTGTCCAGCTCCCC；R：CGCTCAATCGCCCAAA
c59163_g1	F：TTTGGAAGACGAACAA；R：CTGGAGAAGGAGGTGC
c2728_g1	F：TGGTGCTGGCAAGATG；R：ACGCAGTGCTGGTGAA
c7212_g1	F：TCGTCTTCATCCGCCA；R：CCCAACCATAATCCCC
c63240_g1	F：GGGTGGGTTGGATTAT；R：TTGCCTGGGTAGAGTG
c100428_g1	F：CTACTGAGGCTGGCTT；R：GGATTGTCGCTGGAAA
c 42120_g1	F：AACGATCCAGACCAGG；R：TCTCTCAGCAGTGCCA
c404_g1	F：CTAATAACGACCCAAT；R：GCAAAGAACACACAAA
c42905_g1	F：GGTCTTACCACCATCA；R：GGGAACCTTTTCTCTA
c2922_g1	F：CATCCATTGCTTGTCG；R：ATCATTCTGCTCTGCC
c80392_g1	F：CATTGCGTTCATCGTT；R：CAAGCCCCTCTTTTTT
c32780_g1	F：TTCACTGGTTACTCTC；R：AACTCAATCTTGTTCT
c40734_g1	F：CCTCACGACCTTTCAA；R：CTTTCCCCACTCTCTG
c62684_g1	F：GTTGCTCGGCTGTTCG；R：AGTTGATGGTTTGGGA
c65620_g1	F：TTGTAGCAACCAAGGC；R：ACGGAAAAGAAAATCT
ACTIN-7	F：AATCCCAAGGCAAACAGA；R：CCATACCAGAATCCAG

基因名称 Gene name	引物序列（5′-3′） Primers sequence
c19370_g1	F：CGCTGTCCAGCTCCCC；R：CGCTCAATCGCCCAAA
c59163_g1	F：TTTGGAAGACGAACAA；R：CTGGAGAAGGAGGTGC
c2728_g1	F：TGGTGCTGGCAAGATG；R：ACGCAGTGCTGGTGAA
c7212_g1	F：TCGTCTTCATCCGCCA；R：CCCAACCATAATCCCC
c63240_g1	F：GGGTGGGTTGGATTAT；R：TTGCCTGGGTAGAGTG
c100428_g1	F：CTACTGAGGCTGGCTT；R：GGATTGTCGCTGGAAA
c 42120_g1	F：AACGATCCAGACCAGG；R：TCTCTCAGCAGTGCCA
c404_g1	F：CTAATAACGACCCAAT；R：GCAAAGAACACACAAA
c42905_g1	F：GGTCTTACCACCATCA；R：GGGAACCTTTTCTCTA
c2922_g1	F：CATCCATTGCTTGTCG；R：ATCATTCTGCTCTGCC
c80392_g1	F：CATTGCGTTCATCGTT；R：CAAGCCCCTCTTTTTT
c32780_g1	F：TTCACTGGTTACTCTC；R：AACTCAATCTTGTTCT
c40734_g1	F：CCTCACGACCTTTCAA；R：CTTTCCCCACTCTCTG
c62684_g1	F：GTTGCTCGGCTGTTCG；R：AGTTGATGGTTTGGGA
c65620_g1	F：TTGTAGCAACCAAGGC；R：ACGGAAAAGAAAATCT
ACTIN-7	F：AATCCCAAGGCAAACAGA；R：CCATACCAGAATCCAG

基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
镁—原卟啉Ⅸ甲基转移酶 CHLM-magnesium-protoporphyrin Ⅸ methyltransferase	c19370_g1	2.38	上调Up
谷氨酰-tRNA还原酶 HEMA-glutamyl-tRNA reductase	c59163_g1	2.30	上调Up
尿卟啉原脱羧酶 HEME-uroporphyrinogen decarboxylase	c2728_g1	-1.72	下调Down

基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
镁—原卟啉Ⅸ甲基转移酶 CHLM-magnesium-protoporphyrin Ⅸ methyltransferase	c19370_g1	2.38	上调Up
谷氨酰-tRNA还原酶 HEMA-glutamyl-tRNA reductase	c59163_g1	2.30	上调Up
尿卟啉原脱羧酶 HEME-uroporphyrinogen decarboxylase	c2728_g1	-1.72	下调Down

基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
玉米黄素环氧化酶ABA1-zeaxanthin epoxidase	c7212_g1	-3.78	下调Down
类胡萝卜素裂解双加氧酶7 CCD7-carotenoid cleavage dioxygenase 7	c63240_g1	-2.32	下调Down
脱落酸8′-羟化酶1 ABAH1-ABA8’-hydroxylases 1	c100428_g1	-1.69	下调Down
类胡萝卜素裂解双加氧酶8 CCD8-carotenoid cleavage dioxygenase 8	c11404_g1	3.40	上调Up
脱落酸8′-羟化酶3 ABAH3-ABA8’-hydroxylases 3	c23549_g1	1.36	上调Up