https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2023, Vol. 50 ›› Issue (1): 103-115.doi: 10.16420/j.issn.0513-353x.2021-0919

• 研究论文 • 上一篇    下一篇

荷花花器官性状与SSR标记的关联分析

刘艺平1,2, 倪梦辉1, 吴芳芳1, 刘红利1,2, 贺丹1,2, 孔德政1,2,*()   

  1. 1河南农业大学风景园林与艺术学院,郑州 450002
    2河南省优质花卉蔬菜种苗工程技术中心,郑州 450002
  • 收稿日期:2022-09-14 修回日期:2022-11-01 出版日期:2023-01-25 发布日期:2023-01-18
  • 通讯作者: *(E-mail:kzxy303@163.com
  • 基金资助:
    国家自然科学基金项目(31600568);河南省高等学校重点研究项目(21A220003);河南农业大学科技创新基金项目(KJCX2017C01)

Association Analysis of Organ Traits with SSR Markers in Lotus(Nelumbo nucifera

LIU Yiping1,2, NI Menghui1, WU Fangfang1, LIU Hongli1,2, HE Dan1,2, KONG Dezheng1,2,*()   

  1. 1College of Landscape Architecture and Art,Henan Agricultural University,Zhengzhou 450002,China
    2Henan High-quality Flower and Vegetable Seedling Engineering Technology Center,Zhengzhou 450002,China
  • Received:2022-09-14 Revised:2022-11-01 Online:2023-01-25 Published:2023-01-18
  • Contact: *(E-mail:kzxy303@163.com

摘要:

为了挖掘与荷花花器官性状显著相关的分子标记,对60份荷花种质资源的18个花器官性状进行鉴定描述,用筛选得到的16个具有多态性的SSR标记进行遗传多样性分析,并使用TASSEL5.0软件的广义线性模型(general linear mode,GLM)和混合线性模型(mixed linear mode,MLM)对18个花器官性状进行关联分析。研究结果显示,16个SSR标记以二核苷酸重复和三核苷酸重复类型为主,两者占比均为43.75%;在60份荷花种质中扩增出105个多态性位点,平均多态性位点为6.5625;引物多态性含量(PIC)为0.2898 ~ 0.7029,平均值为0.5242;Shannon’s信息指数(I)平均值为1.1548;观测杂合度(Ho)和期望杂合度(He)平均值分别为0.4308和0.5819。60份荷花品种的遗传相似系数(GS)为0.38 ~ 0.92,平均值为0.67;在GS为0.60处将60份荷花种质分为3大种群。GLM模型分析共检测到31个SSR位点,表型解释率为15.72% ~ 76.76%;MLM模型分析共检测到13个SSR位点,表型解释率为20.25% ~ 78.40%;其中有10个位点被二者同时检测到。此外有9个SSR标记的位点在2 ~ 5个花器官性状中被检测到,表现出显著的“一因多效”现象。

关键词: 荷花, 花器官, 性状, SSR分子标记, 关联分析

Abstract:

In order to discover the molecular markers related to the organ traits of lotus flowers,the 18 floral organ traits of 60 lotus germplasm resources were identified and described,16 polymorphic SSR markers were screened to analyze their genetic diversity,and use the generalized linear model(GLM)and mixed linear model(MLM)of TASSEL5.0 software to carry out correlation analysis on 18 traits of floral organs. The results of the study showed that the 16 SSR markers were mainly dinucleotide repeats and trinucleotide repeats,both accounting for 43.75%;105 polymorphic loci were amplified in 60 lotus germplasm materials,with an average number of 6.5625;the primer polymorphism content(PIC)ranged from 0.2898 to 0.7029,with an average value of 0.5242;the average value of Shannon’s information index was 1.1548;and the average value of observed heterozygosity and expected heterozygosity were 0.4308 and 0.5819. The genetic similarity coefficient(GS)of 60 lotus cultivars was from 0.38 to 0.92,with an average value of 0.67. At the GS value of 0.60,60 lotus germplasms were divided into three major populations. GLM model analysis detected 31 SSR loci,with the phenotypic interpretation rate ranged from 15.72% to 76.76%;MLM model analysis detected 13 SSR loci,with a phenotypic interpretation rate ranged from 20.25% to 78.40%;ten of them were detected by both. In addition,nine SSR-labeled loci were detected in 2-5 floral organ traits,showing a significant“one cause and multiple effects”phenomenon.

Key words: lotus, floral organ, trait, SSR molecular marker, association analysis

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