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园艺学报 ›› 2021, Vol. 48 ›› Issue (3): 439-455.doi: 10.16420/j.issn.0513-353x.2020-0421

• 研究论文 • 上一篇    下一篇

梨Trihelix转录因子家族成员鉴定及表达分析

韩文龙1, 朱振1, 李君茹1, 陈天哲1, 李国辉1, 苏雪强1, 金青1,2, 程曦1,2, 蔡永萍1,2,*()   

  1. 1安徽农业大学生命科学学院,合肥 230036
    2地方特色植物资源开发与利用安徽省工程技术中心,合肥 230036
  • 收稿日期:2020-09-28 出版日期:2021-03-25 发布日期:2021-04-02
  • 通讯作者: 蔡永萍 E-mail:swkx12@ahau.edu.cn
  • 基金资助:
    安徽省自然科学基金项目(2008085QC100);安徽农业大学青年基金项目(2019zd01);安徽农业大学稳定和引进人才项目(yj2019-17);安徽省级大学生创新基金项目(201910364156)

Indentification and Expression Analysis of Trihelix Transcription Factor Family in Pear

HAN Wenlong1, ZHU Zhen1, LI Junru1, CHEN Tianzhe1, LI Guohui1, SU Xueqiang1, JIN Qin1,2, CHENG Xi1,2, CAI Yongping1,2,*()   

  1. 1School of Life Science,Anhui Agricultural University,Hefei 230036,China
    2Anhui Provincial Engineering Technology Center for Development & Utilization of Regional Characteristic Plants,Hefei 230036,China
  • Received:2020-09-28 Online:2021-03-25 Published:2021-04-02
  • Contact: CAI Yongping E-mail:swkx12@ahau.edu.cn

摘要:

Trihelix转录因子可以和光应答相关的GT元件结合,因此又称GT转录因子。本研究从梨基因组中鉴定出16个Trihelix家族基因,依次命名为PbGT1 ~ PbGT16,从同属于蔷薇科的草莓和桃基因组中分别鉴定出11和16个Trihelix家族基因。染色体定位与基因复制事件分析表明,梨、草莓和桃Trihelix家族成员分别分布在12、6和8条染色体上,且梨Trihelix家族存在片段复制事件。种间系统进化树分析表明,梨、草莓和桃Trihelix家族成员分为6个亚族,梨家族成员(PbGT)属于GT-2、Subfamily O和SIP1亚族。结合进化关系及qRT-PCR验证,筛选出PbGT15可能参与调控梨果实石细胞团木质化。

关键词: 梨, Trihelix转录因子, 比较基因组学, 表达分析, 木质化

Abstract:

Trihelix transcription factors(TFs)binded to light-responsive GT elements are called GT transcription factors. This study not only identified 16 members of the Trihelix family from the pear genome,named PbGT1-PbGT16 in turn,but also identified 11 and 16 members of the Trihelix family respectively from the genomes of strawberry and peach belonging to the Rosaceae family like pear. Chromosome localization showed that the Trihelix family members of pear,strawberry and peach were distributed on 12,6 and 8 chromosomes,respectively. Segmental duplication events were found in the Trihelix family of pear by gene duplication events analysis. The clustering result of interspecific phylogenetic tree showed that the Trihelix family members of pear,strawberry and peach were divided into six subfamilies and the Trihelix family members of pear(PbGTs) belong to the GT-2,Subfamily O and SIP1 subfamilies. Combined with evolutionary relationship and qRT-PCR verification,PbGT15 may be involved in regulating lignification of pear fruit stone cells.

Key words: pear, Trihelix transcription factors, comparative genomics, expression analysis, lignification

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