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园艺学报 ›› 2012, Vol. 39 ›› Issue (2): 363-369.

• 研究报告 • 上一篇    下一篇

木奈生长发育过程中和受机械损伤后PPO基因的表达

姜翠翠,陈桂信,潘东明,潘腾飞,赖燕,郑鸿昌   

  1. 1福建农林大学园艺学院产品贮存保鲜研究所,福州 350002;2福建农林大学生命科学学院,福州 350002
  • 收稿日期:2011-10-24 修回日期:2012-01-23 出版日期:2012-02-25 发布日期:2012-02-25
  • 通讯作者: 潘东明

Expression of Three Polyphenol Oxidases During Vegetative and Reproductive Development and in Response to Wounding in the Prunus salicina

JIANG Cui-cui,CHEN Gui-xin,PAN Dong-ming,PAN Teng-fei,LAI Yan,ZHENG Hong-chang   

  1. 1Institute of Storage Science and Technology of Horticultural ProductsCollege of HorticultureFujian Agriculture and Forestry UniversityFuzhou 350002China2College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002China
  • Received:2011-10-24 Revised:2012-01-23 Online:2012-02-25 Published:2012-02-25
  • Contact: PAN Dong-ming

摘要: 从(Prunus salicina Lindl. var. cordata J. Y. Zhang et al.)成熟叶片均一化全长cDNA文库中新分离得到了1个PPO基因,命名为PsPPO2,基因登录号为JF681036.1。经NCBI-BLAST分析,其核苷酸序列与蔷薇科果树中的河北鸭梨和富士苹果果实的PPO基因具有很高的同源性,分别为81%和80%;与作者先前分离克隆的PsPPO1PsPPO3基因同源性分别为55%和56%,说明此克隆是PPO基因家族的新成员。通过RT-PCR分析,PsPPO1PsPPO2PsPPO这3个基因在不同生长发育时期和果实受损伤后的表达模式。在叶片发育过程中,PsPPO2表达量都很高;PsPPO1在嫩芽和幼叶中表达;PsPPO3只在嫩芽中表达;在果实发育的不同时期,PsPPO2PsPPO3在早期表达,随着果实成熟表达量下降,PsPPO1在褐变果中表达量较高。受机械损伤后,PsPPO1受诱导,而PsPPO2PsPPO3不受诱导。

关键词: 木奈, 多酚氧化酶, 序列分析, 基因表达, 机械损伤

Abstract:   Polyphenol oxidase(PPO)has been regarded to be a critical enzyme in food technology. A new clone,named PsPPO2,was separated from cDNA library prepared from mature leaf of the Prunus salicina Lindl. var. cordata J. Y. Zhang et al. The deduced amino acid sequences shared 81% and 80% identity with the PPO from Hebei Yali pear and Fuji apple and shared 55% and 56% identity with the PPO gene from P. salicina leaf and fruit which previous isolated,respectively. RT-PCR showed that three PPO gene displayed unique patterns of expression in developmental specific manner in leaf and fruit tissue. In the process of leaf development,the PsPPO2 mRNA was detectable at all stages. In contrast,PsPPO1 and  PsPPO3 were expressed in young tissue. PsPPO2 and PsPPO3 expression are higher at the early stages of fruit development,then dramatically reduced as the fruit ripened. The PsPPO1 mRNA was detectable only at the browning fruit. Upon wounding,PsPPO1 was significantly induced in mature fruit,whereas the level of PsPPO2 and PsPPO3 were not affected by mechanical damage.

Key words: Prunus salicina, polyphenol oxidase, sequence analysis, gene expression, mechanical damage