园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1059-1064.

• 研究简报 • 上一篇    下一篇



  1. 1河北省农林科学院昌黎果树研究所 昌黎 066600;2沈阳农业大学园艺学院,沈阳 110161;3中国农业大学园艺植物研究所 北京 100094)
  • 收稿日期:2008-01-21 修回日期:2008-06-11 出版日期:2008-07-25 发布日期:2008-07-25
  • 通讯作者: 张新忠

SDS-PAGE Analysis of Phase Change-related Proteins in Apple (Malus domestica Borhk.)

ZENG Guang-Juan1,2, LI Chun-Min1, ZHANG Xin-Zhong3*, TIAN Yi2, CHEN Dong-Mei1, ZHAO Yong-Bo1,DONG Wen -Xuan2   

  1. (1Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli Hebei 066600 China;2College of Horticulture, Shenyang Agricultural University, Shenyang 110161,China;3Institute for Horticultural Plants, China Agricultural University, Beijing 100094,China)
  • Received:2008-01-21 Revised:2008-06-11 Online:2008-07-25 Published:2008-07-25
  • Contact: ZHANG Xin-Zhong

摘要: 利用聚丙烯酰胺凝胶电泳技术(SDS-PAGE)分析了6株苹果实生树(‘红玉’ב金冠’)叶片中蛋白质随节位升高的动态变化。结果表明,随节位升高蛋白质带发生变化,共发现了3条阶段转变特异蛋白质带,分子量分别为55 kD、19 kD和17 kD。其中55 kD蛋白质表现为定性变化,有5株实生树在36~75节出现,到101~126节消失;19 kD蛋白质在实生树低节位含量高,在86~100节之后含量突然降低;17 kD蛋白质在实生树低节位含量低,随着植株的发育蛋白质在36~50节含量突然增加并保持稳定,到91~105节后含量突然下降。认为这3种蛋白质是苹果实生树阶段转变的特异蛋白质。

关键词: 苹果, 阶段转变, 蛋白质, SDS-PAGE

Abstract: Dynamic changes of proteins in leaves from different nodes in 6 hybrid seedlings (Jonathan × Golden Delicious) were analyzed with SDS-polyacrylamide gel electrophoresis in apple. Three protein bands were found specific in different phases. The molecular weight of the 3 protein bands were approximately 55 kD, 19 kD and 17 kD. The 55 kD protein presented qualitatively between nodes No.36~75 and No.101~126. The change of 19 kD and 17 kD proteins was found quantitatively. In leaf samples taken below nodes No. 86~100, the content of 19 kD protein was higher, but a sharp decrease occurred in leaves above nodes No.100. The content of 17 kD protein kept high between nodes No. 36~50 and No. 91~105. It is therefore suggested that the 3 proteins were phase change-related in apple seedlings.

Key words: Malus domestica, phase change, protein, SDS-PAGE