https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2022, Vol. 49 ›› Issue (3): 473-481.doi: 10.16420/j.issn.0513-353x.2020-0605

• 研究论文 •    下一篇

适量稳定供锌促进平邑甜茶幼苗生长和氮的吸收利用

于波, 秦嗣军, 吕德国*()   

  1. 沈阳农业大学园艺学院,辽宁省果树品质发育与调控重点实验室,沈阳 110866
  • 收稿日期:2021-09-07 修回日期:2022-01-24 出版日期:2022-03-25 发布日期:2022-03-25
  • 通讯作者: 吕德国 E-mail:lvdeguo@syau.edu.cn
  • 基金资助:
    国家重点研发计划项目(2016YFD0201115);国家现代农业产业技术体系建设专项资金项目(CARS-27);国家自然科学基金项目(31972359)

Continuous Supply of Zinc in Suitable Level Stimulates the Growth and Absorption and Utilization of Nitrogen in Malus hupehensis Seedlings

YU Bo, QIN Sijun, LÜ Deguo*()   

  1. College of Horticulture,Shenyang Agricultural University,Key Laboratory of Fruit Quality Development and Regulation of Liaoning Province,Shenyang 110866,China
  • Received:2021-09-07 Revised:2022-01-24 Online:2022-03-25 Published:2022-03-25
  • Contact: LÜ Deguo E-mail:lvdeguo@syau.edu.cn

摘要:

以苹果砧木平邑甜茶(Malus hupehensis)幼苗为试材,结合15N同位素示踪技术,连续两年研究了砂培条件下低锌、适量和高锌水平(0.5、4和16 μmol · L-1 Zn2+)5种供给方式[持续低锌30 d、低锌变高锌(前15 d低锌,后15 d高锌)、适量稳定供锌(30 d适量供锌)、高锌变低锌(前15 d高锌,后15 d低锌)和持续高锌(30 d高锌)]对幼苗生长以及氮素吸收利用的影响。结果表明:适量稳定供锌处理的幼苗总生物量、根系总长、总表面积最大,根系活力最高;持续低锌处理最低。整个处理期间,适量稳定供锌处理的叶片硝酸还原酶(NR)活性均显著高于其他处理。15N示踪结果表明,处理30 d时,各处理15N吸收量、15N利用率表现为适量稳定供锌处理最高;持续高锌、低锌变高锌处理次之;持续低锌处理最低。以上结果说明:供锌不足与过量以及供锌不稳定均抑制了平邑甜茶幼苗根系生长以及氮素的同化,限制了对氮素的吸收,不利于生长发育;而适量稳定供锌优化了根系形态,同时增强了叶片NR活性,促进了幼苗体内的氮素同化,对氮素的高效吸收利用,幼苗生长发育最有利。

关键词: 苹果, 锌水平, 变换浓度, 氮素, 吸收

Abstract:

Sand culture method was used with Malus hupehensis seedlings as tested materials, 15N isotope tracer was used to study the effects of deficient Zn,moderate Zn and excess Zn(0.5,4 and 16 μmol · L-1 Zn2+)and five methods[keeping in deficient Zn(0.5 μmol · L-1 Zn2+ for 30 d)treatment,Zn concentrations ranged from deficient to excess(0.5 μmol · L-1 Zn2+ 15 d + 16 μmol · L-1Zn2+ 15 d),moderate stable Zn supply(4 μmol · L-1 Zn2+for 30 d)treatment,Zn concentrations ranged from excess to deficient(16 μmol · L-1 Zn2+ 15 d + 0.5 μmol · L-1Zn2+ 15 d)and keeping in excess Zn(16 μmol · L-1 Zn2+ 30 d)] on the growth,nitrogen absorption and utilization. The results of the two consecutive years of testing showed that at the 30th day after the treatment,the plant dry matter,total length and total surface area of root under moderate stable Zn supply treatment were the largest,and root activity was significantly higher than that under other treatments. Nitrate reductase(NR)activity was the highest in moderate stable zinc supply treatment during the whole treatment period. The results of 15N isotope tracer showed that the 15N absorption and 15N utilization under moderate stable zinc supply treatment were the highest,followed by keeping in excess Zn and Zn concentrations ranged from deficient to excess treatment,and the lowest value was founded in keeping in deficient Zn treatment. All these results indicated that insufficient and excessive zinc supply inhibited root growth and nitrogen assimilation,limited nitrogen absorption,and was not conducive to the growth and development of seedlings. Sustained and stable zinc supply can improve root activity and maintain nitrate reductase activity,so as to achieve efficient absorption and utilization of nitrogen,thus promoting the growth of apple rootstock seedlings.

Key words: apple, zinc level, change the concentration, nitrogen, absorption

中图分类号: