关键词: 菜薹, Flowering locus C基因, 表达

Abstract: The morphogenesis of the flower buds of the early and late varieties of flowering Chinese cabbage was found to be almost the same. All flower buds began to differentiate at the 2 to 3 leaf stage. Based on the conserved sequence of Flowering Locus C (FLC) and FRIGIDA (FRI) gene of Arabidopsis thaliana, a 612 bp fragment with an ORF encoding 198 amino acids and a 463 bp cDNA fragment were amplified by RT-PCR from cDNA of flower stalk and were named BrcuFLC (GenBank accession number: EF138603) and BrcuFRI (GenBank accession number: EU700362). Semi-quantitative RT-PCR analysis showed that the expression of BrcuFLC was different in the early and late varieties. With the increase of the leaves, the BrcuFLC expression of two varieties were decreased, but the decrease quantity was not obvious in the late variety. The expression of BrcuFRI was very low in both of two varieties. BrcuFLC would transcript differently at various sites. The intensity of the stem and leave was distinctly more strong than that of flower and root, but for the BrcuFRI expression, the intensity of the root was the most strong in four organs'.

Key words: flowering chinese cabbage（Brassica campestris L. ssp. chinensis (L.) Makino var. utilis Tsen et Lee）, flowering locus C gene, expression