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园艺学报 ›› 2022, Vol. 49 ›› Issue (5): 995-1007.doi: 10.16420/j.issn.0513-353x.2021-0533

• 研究论文 • 上一篇    下一篇

葡萄生长调控因子GRF家族基因的鉴定及表达分析

梁晨, 孙如意, 向锐, 孙艺萌, 师校欣, 杜国强(), 王莉()   

  1. 河北农业大学园艺学院,河北保定 071000
  • 收稿日期:2022-01-27 修回日期:2022-03-28 出版日期:2022-05-25 发布日期:2022-05-25
  • 通讯作者: 杜国强,王莉 E-mail:gdu@hebau.edu.cn;vivi@hebau.edu.cn
  • 基金资助:
    河北省教育厅高等学校科学技术青年基金项目(QN2020232);河北省青年科学基金项目(C2021204146);现代种业科技创新专项(21326310D);河北省现代农业产业技术体系项目(HBCT2018100204)

Genome-wide Identification of Grape GRF Family and Expression Analysis

LIANG Chen, SUN Ruyi, XIANG Rui, SUN Yimeng, SHI Xiaoxin, DU Guoqiang(), WANG Li()   

  1. College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Received:2022-01-27 Revised:2022-03-28 Online:2022-05-25 Published:2022-05-25
  • Contact: DU Guoqiang,WANG Li E-mail:gdu@hebau.edu.cn;vivi@hebau.edu.cn

摘要:

开展葡萄生长调控因子GRF(Growth-regulating factor)家族成员的基因结构、蛋白保守基序、亚细胞定位预测、同线性、系统进化树及顺式作用元件等分析;利用qRT-PCR技术开展有核和无核葡萄不同组织器官、种子发育不同时期及激素响应表达模式分析。葡萄GRF家族共有8个成员,其中7个分布于6条染色体,编码氨基酸数为213 ~ 604,所有成员均定位于细胞核。根据进化关系分为4组(A ~ D),同组VvGRF的外显子—内含子结构、保守基序数和类型均具有保守性。所有葡萄GRF蛋白N端均含有QLQ和WRC保守结构域,C端至少含有TQL或FFD结构域之一。同线性分析发现,VvGRF3VvGRF4存在并联重复。VvGRF启动子区发现大量与生长发育、激素响应及胁迫响应相关顺式作用元件。大部分VvGRF在营养器官叶片中高表达,VvGRF2在生殖器官花和果实中高表达;VvGRF3VvGRF6在无核葡萄种子发育过程中表达量显著高于有核葡萄,而VvGRF8在有核葡萄种子发育前期表达量显著高于无核葡萄;VvGRF响应GA3和IAA诱导,且大部分基因在处理0.5或1 h后下调。

关键词: 葡萄, GRF, 家族进化, 种子发育, GA3, IAA, 表达分析

Abstract:

The gene structure analysis,protein conserved motif identification,subcellular localization prediction,synteny analysis,phylogenetic analysis,and cis-acting elements analysis of Growth-regulating factor(GRF)family members were performed. Expression patterns of grape GRF genes in different plant parts and against different hormone applications were analyzed. Further expression patterns were compared during different stages of seed development in seeded and seedless cultivars. Eight grape GRF genes were identified and divided into four groups(A-D). Seven of the genes were unevenly distributed on six chromosomes having and the number of amino acids was 213-604. All grape GRF proteins were predicted in the nucleus. Moreover,VvGRFs in the same clade showed conserved exon-intron structure and motif distribution patterns. All GRF proteins contained QLQ and WRC domains in the N-terminal region and at least one TQL or FFD domain in the C-terminal region. Synteny analysis showed that VvGRF3 and VvGRF4 were segmentally duplicated. Various cis-acting elements related to growth and development,hormones response,and stresses were found in the promoter region of VvGRF genes. Expression analysis illustrated that most of the VvGRFs were highly expressed in vegetative organs i.e. leaves. However,VvGRF2 was highly expressed in reproductive organs like flowers and fruits. The expression of VvGRF3 and VvGRF6 was significantly higher during seed development in seedless grapes as compared to seeded grapes,while VvGRF8 showed high expression in seeded grapes. The expression of VvGRF genes was induced against GA3 and IAA treatments,and most of the VvGRFs were down-regulated at 0.5 or 1 h.

Key words: grape, GRF, family evolution, seed development, GA3, IAA, expression analysis

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