γ-射线对苹果和梨离体叶片不定梢再生及芽生长的影响

园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 217-220.

γ-射线对苹果和梨离体叶片不定梢再生及芽生长的影响

孙清荣;李林光;罗圣华;刘鹏;杨建明

(山东省果树研究所，泰安 271000)

收稿日期:2003-04-07 修回日期:2003-10-18 出版日期:2004-04-25 发布日期:2004-04-25

The Effct of Gamma Irradiation on Shoot Regeneration from Leaf and BudGrowth of Pear and Apple in Vitro

Sun Qingrong;Li Linguang;Fan Shenghua;Liu Peng;Yang Jianming

(Shandong Institute of Pomology，Tai'an 271000，China)

Received:2003-04-07 Revised:2003-10-18 Online:2004-04-25 Published:2004-04-25

摘要/Abstract

摘要： 用γ-射线照射苹果和梨的无根无菌苗，研究其对叶片不定梢再生及芽生长的影响。对照射
最敏感的器官是叶片，其次是未萌发的侧芽，第三是正在生长的顶芽。叶片不定梢再生的半致死剂量为20~30 Gy，苹果侧芽萌发的半致死剂量为30 Gy，梨为30 Gy以上。照射剂量在10—30 Gy时，抑制顶芽的伸长生长，但不引起致死或其他不良生长症状。

关键词: 苹果, 梨, 叶片, 芽, γ-射线, 组织培养

Abstract: The shoots of pear and apple in vitro were irradiated by gamma rays． The effects of gamma irradiation on shoot regeneration from leaf and bud growth were studied． The organ which is the most sensitive to γ-rays was leaf，second was unbudding lateral bud， the third was growing shoot tip． The LD₅₀(50% decrease of regeneration)of leaf was genotype-dependent and was between 20 and 30 grays．The LD₅₀ of lateral bud also was genotype-dependent and was 30 grays or above 30 grays． Doses of iradiation were between 10 and 30 grays，the elongation growth of shoot tip was inhibited， but no lethality or other symptoms．
Key words：

Key words: Apple, Pear, Leaf, Bud, γ-rays, Tissue culture

中图分类号:

孙清荣;李林光;罗圣华;刘鹏;杨建明. γ-射线对苹果和梨离体叶片不定梢再生及芽生长的影响[J]. 园艺学报, 2004, 31(2): 217-220.

Sun Qingrong;Li Linguang;Fan Shenghua;Liu Peng;Yang Jianming. The Effct of Gamma Irradiation on Shoot Regeneration from Leaf and BudGrowth of Pear and Apple in Vitro[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 217-220.

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