图1 蜡梅（Cp）、拟南芥（At）、水稻（At）和梅花（Pm）ABCG蛋白的系统进化树

Fig. 1 Phylogenetic tree of ABCG proteins in Chimonanthus praecox（Cp），Arabidopsis thaliana（At），Oryza sativa（At）and Prunus mume（Pm）

图2 CpABCG基因家族保守基序及基因结构分析 WBC型和PDR型的基因分别用蓝色线条和红色线条表示

Fig. 2 Conserved motif and gene structure of CpABCG gene family The WBC and PDR genes are represented by blue and red lines，respectively

图3 蜡梅 ABCG 基因家族染色体定位 具有片段复制的CpABCG基因用红色字体表示，串联复制以红线表示

Fig. 3 Chromosomal localizationof the CpABCG family members in Chimonanthus praecox CpABCG genes with fragment duplication are shown in red fonts，and tandem duplication is shown in red lines

图4 蜡梅ABCG基因复制分析 外侧方框代表染色体骨架，中间和内侧方框表示基因密度，每个基因在染色体骨架上的大致分布用短黑线标出，红色线表示CpABCG的片段复制基因对

Fig. 4 Replication events of the CpABCG family members in Chimonanthus praecox The outer box represents the chromosome skeleton，the middle and inner boxes indicate gene density，and the approximate distribution of each CpABCG gene is marked on the chromosome skeleton by a short black line，the red line indicates the segmental duplication gene pairs of the CpABCG

图5 CpABCG基因不同物种间共线性分析 背景中灰色线条表示蜡梅与其他物种之间的共线性区块，红色线条突出了CpABCG基因对

Fig. 5 Covariance analysis of CpABCG genes in different species Gray lines indicate blocks of covariance between Chimonanthus praecox and other species，and red lines highlight CpABCG gene pairs

图6 CpABCG基因家族启动子顺式作用元件分析 WBC型和PDR型的基因分别用蓝色线条和红色线条表示

Fig. 6 Analysis of cis-acting elements of CpABCG gene family promoters The WBC and PDR genes are represented by blue and red lines，respectively

图7 转录组中CpABCG基因表达情况热图

Fig. 7 Heat map of CpABCG expression in transcriptome data

图8 蜡梅花香相关ABCG基因进化树

Fig. 8 Floral related CpABCG phylogenetic tree

图9 候选CpABCG基因不同部位不同时间相对表达量分析 邓肯氏单因素方差分析，不同小写字母表示各样本间差异显著（P < 0.05）

Fig. 9 Analysis of relative expression of candidate CpABCG gene in different parts and time One-way ANOVA，Duncan’s test. Different lowercase letters indicate significant among different samples at 0.05 level

图10 外部释放和内源花香总量 t检验。**，***分别表示处理间差异显著（P < 0.01，P < 0.001）

Fig. 10 Comparison of total amount of external release and endogenous flower fragrance between experimental group and control group t-test. **，*** indicate significant differences（P < 0.01，P < 0.001）

图11 主要花香成分外部挥发情况 t检验。**，***和ns分别表示处理间的差异显著性（P < 0.01，P < 0.001，P > 0.05）。下同

Fig. 11 External volatilization of main floral components t-test. **，***，and ns indicate significant differences between treatments，respectively（P < 0.01，P < 0.001，P > 0.05）. The same below

图12 主要花香成分内源含量

Fig. 12 Endogenous content of main floral components

图13 在外源花香抑制剂处理后候选基因的qRT-PCR检测水平

Fig. 13 Quantitative analysis of candidate genes after treated with exogenous flowe inhibitor