图1 VaSTS19和VdSTS19不同长度启动子片段 D1 ~ D5表示VaSTS19不同长度启动子片段；S1 ~ S5表示VdSTS19不同长度启动子片段。下同。

Fig. 1 Schematic representation of promoter fragments of different lengths of VaSTS19 and VdSTS19 D1-D5 indicate the promoter fragments of different lengths of VaSTS19；S1-S5 indicate the promoter fragments of different lengths of VdSTS19. The same below.

图2 STS家族基因在葡萄不同组织器官（A）和不同发育时期果实（B）中的表达 A：1 ~ 8分别为‘通化3号’根、茎、叶、花序、果皮、果肉、种子和卷须； B：1 ~ 3分别为‘塘尾’绿果期，转色期和成熟期果实；4 ~ 6为‘通化3号’绿果期、转色期和成熟期果实。

Fig. 2 Tissue specific expression profile analysis (A) and fruit development period expression (B) of STS genes A：Vitis amurensis‘Tonghua 3’. 1，root；2，stem；3，leaf；4，flower；5，pericarp；6，flesh；7，seed；8，tendril. B：1-3：Grapevine fruit of green，veraison and mature stages in V. davidii‘Tangwei’；4-6：Grapevine fruit of green，veraison and mature stages in V. amurensis‘Tonghua 3’.

图3 VaSTS19和VdSTS19蛋白的亚细胞定位

Fig. 3 Subcellular localization of VaSTS19 and VdSTS19 proteins

图4 番茄的转基因遗传转化过程（A）和PCR扩增鉴定转化番茄植株（B） a：种子萌发长出子叶；b：子叶预培养；c：侵染后共培养；d：转化后形成的愈伤组织；e：产生的抗性芽；f和g：抗性芽生根；h：移栽。M：DNA marker DL2000；1 ~ 6：转化VaSTS19的转基因株系；7 ~ 12：转化VdSTS19的转基因株系；13 ~ 17：转化空载体2300的转基因株系；18、19：野生型株系。

Fig. 4 Transformation process of transgenic tomatos（A）and PCR verifies the recombinant plasmid in transformed tomato（B） a：Cotyledon germination；b：Cotyledon precultured；c：Co-culture after infection；d：Callus formed after transformation；e：Resistant shoots generate；f and g：Resistant bud rooting；h：Transplanting to the greenhouse. M：Indicates DNA marker DL 2000；Lane 1-6：Indicate the transgenic plants of VaSTS19；Lane 7-12：Indicate the transgenic plants of VdSTS19；Lane 13-17：Indicate the transgenic plants of 2300；Lane 18，19：Indicate wild type tomato.

图5 VaSTS19和VdSTS19的启动子的序列比对 红色方框表示转录起始位点（+1）；背景绿色的元件代表两个启动子序列中所共有的元件，粉色和红色分别表示‘通化3号’和‘塘尾’STS19启动子序列上特有元件。方向向左的箭头表明所标注元件与启动子方向相反；向下或向上的箭头表示启动子缺失体的起始位点。

Fig. 5 A comparison of the VaSTS19 and VdSTS19 promoter sequences The translational start sites（+1）are shown in red box. Motifs with significant similarity to previously identified cis-acting elements in both species are shaded in green. Motifs found only in VaSTS19 or VdSTS19 promoters are respectively shaded in pink or red. Arrowheads pointing to the left represent the reverse direction of cis-elements compared to promoter orientation. Other arrows represent start point of 5′-deleted derivatives.

图6 不同葡萄种间芪合成酶基因启动子序列的聚类 标尺代表每单位长度发生0.05次剪辑替换，结点处的数字代表自展值（1 000次重复）。

Fig. 6 A phylogenetic tree based on a ClustalX multiple sequence alignment of STS promoters from a range of grape species The scale bar represents 0.05 substitutions per site and the numbers next to the nodes are bootstrap values from 1 000 replicates.

图7 不同激素处理下VaSTS19和VdSTS19不同长度启动子片段GUS活性分析 GUS活性值来自3次不同的重复试验。WT表示野生型，N表示阴性对照（不含启动子），35S表示阳性对照（内含CaMV35S启动子）。两组间的显著性分析采用单侧t检测进行分析，*表示显著（α = 0.05），**极显著（α = 0.01）。

Fig. 7 GUS activity analysis of promoter fragments of different lengths of VaSTS19 and VdSTS19 in response to hormone treatment in transiently transformed tobacco leaves Mean GUS activity（± SE）is averaged from triplicate experiments. WT：Wild type；N：Negative control（no promoter）；35S：Positive control （CaMV35S promoter）. Significant difference test were evaluated using a one-sided paired t test（**α = 0.01，or at *α = 0.05）.