园艺学报 ›› 2023, Vol. 50 ›› Issue (6): 1295-1304.doi: 10.16420/j.issn.0513-353x.2022-0368
收稿日期:
2022-11-27
修回日期:
2023-03-03
出版日期:
2023-06-25
发布日期:
2023-06-27
作者简介:
* E-mail:qsj1975@syau.edu.cn
基金资助:
QIN Sijun*(), ZHANG Kuo, QI Bianbin, YU Bo, LÜ Deguo
Received:
2022-11-27
Revised:
2023-03-03
Published:
2023-06-25
Online:
2023-06-27
摘要:
以辽中地区较为典型的低碳砂质果园土壤为栽培基质,以‘寒富’苹果/山荆子幼苗(Malus × domesica‘Hanfu’/M. baccata)为试材,以有机物料分解最终产物之一且易被吸收利用的葡萄糖作为碳源,通过设置添加葡萄糖及土壤灭菌处理,研究苹果根域土壤活性有机碳库组分和根系呼吸等生理代谢过程对外源碳输入的响应。结果表明,土壤添加葡萄糖和灭菌后添加葡萄糖处理整体上提高了土壤有机碳、微生物量碳、水溶性有机碳和颗粒有机碳含量,而单纯土壤灭菌处理则降低了土壤有机碳、微生物量碳和颗粒有机碳含量。添加葡萄糖和灭菌后添加葡萄糖处理显著提高了土壤纤维素酶和β-葡萄糖苷酶活性,提高了苹果植株根系参与呼吸代谢途径中磷酸烯醇式丙酮酸羧化酶、异柠檬酸脱氢酶和苹果酸脱氢酶的活性,并且增强光合能力,促进了植株生长。综上,在低碳砂质土壤中,按照土壤微生物碳本底水平添加5倍量的易分解碳源有利于提高土壤活性碳库组分含量,增强土壤酶活性,改善土壤质量,同时强化了苹果叶片光合能力,并最终促进植株生长发育。
中图分类号:
秦嗣军, 张阔, 齐边斌, 于波, 吕德国. 外源碳对苹果根区土壤活性有机碳及植株生长的影响[J]. 园艺学报, 2023, 50(6): 1295-1304.
QIN Sijun, ZHANG Kuo, QI Bianbin, YU Bo, LÜ Deguo. Effects of Exogenous Glucose on Soil Active Organic Carbon Pool in Apple Root Zone and Plant Growth and Development[J]. Acta Horticulturae Sinica, 2023, 50(6): 1295-1304.
时间/d | 处理Treatment | 有机碳/(g · kg-1)SOC | 微生物量碳/ (mg · kg-1) MBC | 水溶性有机碳/ (mg · kg-1) DOC | 颗粒有机碳/ (g · kg-1) POC |
---|---|---|---|---|---|
3 | 对照Control | 4.02 ± 0.06 c | 213.55 ± 26.78 c | 70.13 ± 6.15 c | 0.43 ± 0.03 c |
添加葡萄糖Adding glucose | 4.60 ± 0.08 b | 330.96 ± 19.44 b | 120.80 ± 6.21 b | 0.47 ± 0.01 b | |
灭菌Sterilization | 3.80 ± 0.09 d | 136.80 ± 12.21 d | 65.01 ± 3.56 c | 0.35 ± 0.02 d | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.80 ± 0.14 a | 392.72 ± 14.53 a | 130.85 ± 6.35 a | 0.51 ± 0.01 a | |
7 | 对照Control | 3.93 ± 0.08 c | 254.82 ± 16.56 c | 65.30 ± 3.69 c | 0.43 ± 0.02 b |
添加葡萄糖Adding glucose | 4.43 ± 0.09 b | 415.35 ± 17.61 a | 107.70 ± 10.83 b | 0.48 ± 0.02 a | |
灭菌Sterilization | 3.70 ± 0.05 d | 146.23 ± 12.93 d | 58.56 ± 4.63 c | 0.35 ± 0.01 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.62 ± 0.15 a | 352.15 ± 17.58 b | 117.34 ± 8.34 a | 0.50 ± 0.01 a | |
15 | 对照Control | 3.99 ± 0.06 b | 240.26 ± 16.16 b | 61.39 ± 3.65 c | 0.43 ± 0.02 c |
添加葡萄糖Adding glucose | 4.32 ± 0.13 a | 326.01 ± 20.11 a | 82.62 ± 5.43 b | 0.56 ± 0.02 a | |
灭菌Sterilization | 3.74 ± 0.04 c | 158.11 ± 19.13 c | 57.08 ± 2.37 c | 0.36 ± 0.03 d | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.40 ± 0.15 a | 304.25 ± 15.63 a | 97.30 ± 3.28 a | 0.51 ± 0.02 b | |
30 | 对照Control | 3.96 ± 0.08 b | 270.83 ± 12.45 b | 58.03 ± 8.31 b | 0.42 ± 0.03 b |
添加葡萄糖Adding glucose | 4.29 ± 0.11 a | 307.37 ± 16.37 a | 77.89 ± 6.13 a | 0.58 ± 0.02 a | |
灭菌Sterilization | 3.63 ± 0.03 c | 152.15 ± 9.12 c | 64.18 ± 5.28 b | 0.36 ± 0.02 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.39 ± 0.15 a | 302.94 ± 13.31 a | 85.90 ± 3.25 a | 0.56 ± 0.02 a | |
60 | 对照Control | 3.87 ± 0.04 b | 264.91 ± 9.46 b | 63.81 ± 5.28 b | 0.41 ± 0.01 b |
添加葡萄糖Adding glucose | 4.18 ± 0.09 a | 303.99 ± 15.43 a | 65.49 ± 4.63 b | 0.63 ± 0.01 a | |
灭菌Sterilization | 3.62 ± 0.02 c | 169.91 ± 8.59 c | 53.94 ± 2.31 c | 0.36 ± 0.03 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.30 ± 0.14 a | 277.89 ± 21.33 ab | 83.05 ± 4.21 a | 0.61 ± 0.03 a |
表1 苹果根区土壤不同处理对活性有机碳库组分含量的影响
Table 1 Effects of adding glucose on the content of soil active organic carbon pool in apple root zone
时间/d | 处理Treatment | 有机碳/(g · kg-1)SOC | 微生物量碳/ (mg · kg-1) MBC | 水溶性有机碳/ (mg · kg-1) DOC | 颗粒有机碳/ (g · kg-1) POC |
---|---|---|---|---|---|
3 | 对照Control | 4.02 ± 0.06 c | 213.55 ± 26.78 c | 70.13 ± 6.15 c | 0.43 ± 0.03 c |
添加葡萄糖Adding glucose | 4.60 ± 0.08 b | 330.96 ± 19.44 b | 120.80 ± 6.21 b | 0.47 ± 0.01 b | |
灭菌Sterilization | 3.80 ± 0.09 d | 136.80 ± 12.21 d | 65.01 ± 3.56 c | 0.35 ± 0.02 d | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.80 ± 0.14 a | 392.72 ± 14.53 a | 130.85 ± 6.35 a | 0.51 ± 0.01 a | |
7 | 对照Control | 3.93 ± 0.08 c | 254.82 ± 16.56 c | 65.30 ± 3.69 c | 0.43 ± 0.02 b |
添加葡萄糖Adding glucose | 4.43 ± 0.09 b | 415.35 ± 17.61 a | 107.70 ± 10.83 b | 0.48 ± 0.02 a | |
灭菌Sterilization | 3.70 ± 0.05 d | 146.23 ± 12.93 d | 58.56 ± 4.63 c | 0.35 ± 0.01 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.62 ± 0.15 a | 352.15 ± 17.58 b | 117.34 ± 8.34 a | 0.50 ± 0.01 a | |
15 | 对照Control | 3.99 ± 0.06 b | 240.26 ± 16.16 b | 61.39 ± 3.65 c | 0.43 ± 0.02 c |
添加葡萄糖Adding glucose | 4.32 ± 0.13 a | 326.01 ± 20.11 a | 82.62 ± 5.43 b | 0.56 ± 0.02 a | |
灭菌Sterilization | 3.74 ± 0.04 c | 158.11 ± 19.13 c | 57.08 ± 2.37 c | 0.36 ± 0.03 d | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.40 ± 0.15 a | 304.25 ± 15.63 a | 97.30 ± 3.28 a | 0.51 ± 0.02 b | |
30 | 对照Control | 3.96 ± 0.08 b | 270.83 ± 12.45 b | 58.03 ± 8.31 b | 0.42 ± 0.03 b |
添加葡萄糖Adding glucose | 4.29 ± 0.11 a | 307.37 ± 16.37 a | 77.89 ± 6.13 a | 0.58 ± 0.02 a | |
灭菌Sterilization | 3.63 ± 0.03 c | 152.15 ± 9.12 c | 64.18 ± 5.28 b | 0.36 ± 0.02 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.39 ± 0.15 a | 302.94 ± 13.31 a | 85.90 ± 3.25 a | 0.56 ± 0.02 a | |
60 | 对照Control | 3.87 ± 0.04 b | 264.91 ± 9.46 b | 63.81 ± 5.28 b | 0.41 ± 0.01 b |
添加葡萄糖Adding glucose | 4.18 ± 0.09 a | 303.99 ± 15.43 a | 65.49 ± 4.63 b | 0.63 ± 0.01 a | |
灭菌Sterilization | 3.62 ± 0.02 c | 169.91 ± 8.59 c | 53.94 ± 2.31 c | 0.36 ± 0.03 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 4.30 ± 0.14 a | 277.89 ± 21.33 ab | 83.05 ± 4.21 a | 0.61 ± 0.03 a |
时间/d Time | 处理 Treatment | β-葡萄糖苷酶/ (p-nitrophenol μg · g-1 · h-1) β-Glycosidase | 纤维素酶/ (glucose mg ·g-1· d-1) Cellulase |
---|---|---|---|
3 | 对照Control | 18.06 ± 0.48 b | 0.075 ± 0.001 b |
添加葡萄糖Adding glucose | 37.97 ± 1.28 a | 0.693 ± 0.011 a | |
灭菌Sterilization | 16.44 ± 1.20 b | 0.081 ± 0.010 b | |
灭菌后添加葡萄糖Sterilization and add glucose | 35.52 ± 3.12 a | 0.742 ± 0.010 a | |
7 | 对照Control | 18.60 ± 0.49 c | 0.103 ± 0.001 c |
添加葡萄糖Adding glucose | 45.39 ± 5.41 a | 0.199 ± 0.007 a | |
灭菌Sterilization | 16.92 ± 1.10 c | 0.099 ± 0.001 c | |
灭菌后添加葡萄糖Sterilization and add glucose | 41.13 ± 1.88 b | 0.153 ± 0.017 b | |
15 | 对照Control | 19.57 ± 2.99 d | 0.070 ± 0.006 b |
添加葡萄糖Adding glucose | 53.33 ± 9.77 a | 0.106 ± 0.003 a | |
灭菌Sterilization | 28.67 ± 2.39 c | 0.094 ± 0.006 ab | |
灭菌后添加葡萄糖Sterilization and add glucose | 43.45 ± 5.34 b | 0.091 ± 0.003 ab | |
30 | 对照Control | 18.42 ± 1.26 c | 0.072 ± 0.017 a |
添加葡萄糖Adding glucose | 57.33 ± 8.31 a | 0.069 ± 0.006 a | |
灭菌Sterilization | 16.09 ± 1.50 c | 0.061 ± 0. 008 a | |
灭菌后添加葡萄糖Sterilization and add glucose | 40.61 ± 7.45 b | 0.077 ± 0.016 a | |
60 | 对照Control | 16.83 ± 2.56 c | 0.066 ± 0.004 ab |
添加葡萄糖Adding glucose | 42.29 ± 3.39 a | 0.065 ± 0.012 ab | |
灭菌Sterilization | 17.51 ± 3.10 c | 0.058 ± 0.003 b | |
灭菌后添加葡萄糖Sterilization and add glucose | 31.38 ± 3.12 b | 0.074 ± 0.007 a |
表2 土壤添加葡萄糖和灭菌处理对苹果根区土壤酶活性的影响
Table 2 Effects of adding glucose and soil sterilization on soil enzyme activities in apple root zone
时间/d Time | 处理 Treatment | β-葡萄糖苷酶/ (p-nitrophenol μg · g-1 · h-1) β-Glycosidase | 纤维素酶/ (glucose mg ·g-1· d-1) Cellulase |
---|---|---|---|
3 | 对照Control | 18.06 ± 0.48 b | 0.075 ± 0.001 b |
添加葡萄糖Adding glucose | 37.97 ± 1.28 a | 0.693 ± 0.011 a | |
灭菌Sterilization | 16.44 ± 1.20 b | 0.081 ± 0.010 b | |
灭菌后添加葡萄糖Sterilization and add glucose | 35.52 ± 3.12 a | 0.742 ± 0.010 a | |
7 | 对照Control | 18.60 ± 0.49 c | 0.103 ± 0.001 c |
添加葡萄糖Adding glucose | 45.39 ± 5.41 a | 0.199 ± 0.007 a | |
灭菌Sterilization | 16.92 ± 1.10 c | 0.099 ± 0.001 c | |
灭菌后添加葡萄糖Sterilization and add glucose | 41.13 ± 1.88 b | 0.153 ± 0.017 b | |
15 | 对照Control | 19.57 ± 2.99 d | 0.070 ± 0.006 b |
添加葡萄糖Adding glucose | 53.33 ± 9.77 a | 0.106 ± 0.003 a | |
灭菌Sterilization | 28.67 ± 2.39 c | 0.094 ± 0.006 ab | |
灭菌后添加葡萄糖Sterilization and add glucose | 43.45 ± 5.34 b | 0.091 ± 0.003 ab | |
30 | 对照Control | 18.42 ± 1.26 c | 0.072 ± 0.017 a |
添加葡萄糖Adding glucose | 57.33 ± 8.31 a | 0.069 ± 0.006 a | |
灭菌Sterilization | 16.09 ± 1.50 c | 0.061 ± 0. 008 a | |
灭菌后添加葡萄糖Sterilization and add glucose | 40.61 ± 7.45 b | 0.077 ± 0.016 a | |
60 | 对照Control | 16.83 ± 2.56 c | 0.066 ± 0.004 ab |
添加葡萄糖Adding glucose | 42.29 ± 3.39 a | 0.065 ± 0.012 ab | |
灭菌Sterilization | 17.51 ± 3.10 c | 0.058 ± 0.003 b | |
灭菌后添加葡萄糖Sterilization and add glucose | 31.38 ± 3.12 b | 0.074 ± 0.007 a |
时间/d Time | 处理 Treatment | 磷酸烯醇式丙酮酸羧化酶/ (μmol · min-1 · g-1FW) PEPC | 苹果酸脱氢酶/ (μmol · min-1 · g-1FW) MDH | 异柠檬酸脱氢酶/ (μmol · min-1 · g-1FW) IDH |
---|---|---|---|---|
3 | 对照Control | 43.62 ± 8.07 c | 64.31 ± 7.17 c | 76.15 ± 4.33 c |
添加葡萄糖Adding glucose | 59.15 ± 5.87 b | 104.75 ± 6.61 b | 146.43 ± 6.85 b | |
灭菌Sterilization | 41.16 ± 6.35 c | 61.24 ± 4.22 c | 43.15 ± 1.91 d | |
灭菌后添加葡萄糖 Sterilization and add glucose | 74.81 ± 8.78 a | 123.60 ± 14.12 a | 179.41 ± 12.50 a | |
7 | 对照Control | 43.64 ± 2.76 c | 67.13 ± 8.34 c | 63.38 ± 4.34 c |
添加葡萄糖Adding glucose | 60.94 ± 3.94 b | 129.52 ± 11.28 b | 121.16 ± 10.61 b | |
灭菌Sterilization | 30.07 ± 5.41 d | 67.05 ± 8.92 c | 56.38 ± 5.83 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 73.68 ± 7.15 a | 146.43 ± 18.73 a | 163.68 ± 12.81 a | |
15 | 对照Control | 47.65 ± 3.17 b | 68.01 ± 7.80 c | 47.68 ± 3.65 c |
添加葡萄糖Adding glucose | 66.01 ± 7.16 a | 95.65 ± 6.06 b | 100.47 ± 13.88 b | |
灭菌Sterilization | 32.99 ± 2.37 c | 63.34 ± 3.63 c | 51.86 ± 8.89 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 69.89 ± 3.63 a | 116.88 ± 16.31 a | 121.86 ± 10.74 a | |
30 | 对照Control | 45.77 ± 7.27 c | 64.31 ± 8.27 c | 55.54 ± 8.65 c |
添加葡萄糖Adding glucose | 57.41 ± 7.05 b | 76.06 ± 7.86 b | 89.43 ± 3.04 b | |
灭菌Sterilization | 21.79 ± 4.50 d | 49.07 ± 3.51 d | 66.99 ± 1.41 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 63.92 ± 4.57 a | 88.11 ± 4.67 a | 123.09 ± 19.17 a | |
60 | 对照Control | 43.43 ± 5.14 c | 66.94 ± 9.18 b | 63.51 ± 8.71 c |
添加葡萄糖Adding glucose | 55.36 ± 8.99 b | 74.29 ± 3.51 a | 72.27 ± 8.02 b | |
灭菌Sterilization | 20.69 ± 4.63 d | 55.87 ± 3.43 b | 64.22 ± 2.88 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 64.30 ± 8.91 a | 72.94 ± 5.03 a | 94.37 ± 7.11 a |
表3 土壤添加葡萄糖和灭菌处理对苹果植株根系呼吸代谢酶活性的影响
Table 3 Effects of adding glucose and soil sterilization on enzyme activities of apple root respiration metabolism
时间/d Time | 处理 Treatment | 磷酸烯醇式丙酮酸羧化酶/ (μmol · min-1 · g-1FW) PEPC | 苹果酸脱氢酶/ (μmol · min-1 · g-1FW) MDH | 异柠檬酸脱氢酶/ (μmol · min-1 · g-1FW) IDH |
---|---|---|---|---|
3 | 对照Control | 43.62 ± 8.07 c | 64.31 ± 7.17 c | 76.15 ± 4.33 c |
添加葡萄糖Adding glucose | 59.15 ± 5.87 b | 104.75 ± 6.61 b | 146.43 ± 6.85 b | |
灭菌Sterilization | 41.16 ± 6.35 c | 61.24 ± 4.22 c | 43.15 ± 1.91 d | |
灭菌后添加葡萄糖 Sterilization and add glucose | 74.81 ± 8.78 a | 123.60 ± 14.12 a | 179.41 ± 12.50 a | |
7 | 对照Control | 43.64 ± 2.76 c | 67.13 ± 8.34 c | 63.38 ± 4.34 c |
添加葡萄糖Adding glucose | 60.94 ± 3.94 b | 129.52 ± 11.28 b | 121.16 ± 10.61 b | |
灭菌Sterilization | 30.07 ± 5.41 d | 67.05 ± 8.92 c | 56.38 ± 5.83 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 73.68 ± 7.15 a | 146.43 ± 18.73 a | 163.68 ± 12.81 a | |
15 | 对照Control | 47.65 ± 3.17 b | 68.01 ± 7.80 c | 47.68 ± 3.65 c |
添加葡萄糖Adding glucose | 66.01 ± 7.16 a | 95.65 ± 6.06 b | 100.47 ± 13.88 b | |
灭菌Sterilization | 32.99 ± 2.37 c | 63.34 ± 3.63 c | 51.86 ± 8.89 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 69.89 ± 3.63 a | 116.88 ± 16.31 a | 121.86 ± 10.74 a | |
30 | 对照Control | 45.77 ± 7.27 c | 64.31 ± 8.27 c | 55.54 ± 8.65 c |
添加葡萄糖Adding glucose | 57.41 ± 7.05 b | 76.06 ± 7.86 b | 89.43 ± 3.04 b | |
灭菌Sterilization | 21.79 ± 4.50 d | 49.07 ± 3.51 d | 66.99 ± 1.41 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 63.92 ± 4.57 a | 88.11 ± 4.67 a | 123.09 ± 19.17 a | |
60 | 对照Control | 43.43 ± 5.14 c | 66.94 ± 9.18 b | 63.51 ± 8.71 c |
添加葡萄糖Adding glucose | 55.36 ± 8.99 b | 74.29 ± 3.51 a | 72.27 ± 8.02 b | |
灭菌Sterilization | 20.69 ± 4.63 d | 55.87 ± 3.43 b | 64.22 ± 2.88 c | |
灭菌后添加葡萄糖 Sterilization and add glucose | 64.30 ± 8.91 a | 72.94 ± 5.03 a | 94.37 ± 7.11 a |
处理Treatment | 净光合速率Pn | 蒸腾速率Tr | 气孔导度Gs |
---|---|---|---|
对照Control | 13.6 ± 1.1 c | 3.5 ± 0.7 b | 232.6 ± 70.4 c |
添加葡萄糖Adding glucose | 19.3 ± 0.4 ab | 3.9 ± 0.1 a | 293.3 ± 56.5 b |
灭菌Sterilization | 16.3 ± 0.5 b | 3.4 ± 0.2 b | 295.3 ± 10.9 b |
灭菌后添加葡萄糖Sterilization and add glucose | 21.2 ± 1.1 a | 4.2 ± 0.3 a | 326.3 ± 36.4 a |
表4 土壤添加葡萄糖和灭菌对苹果植株光合参数的影响
Table 4 Effects of adding glucose and soil sterilization on photosynthetic parameters of apple plants μmol · m-2· s-1
处理Treatment | 净光合速率Pn | 蒸腾速率Tr | 气孔导度Gs |
---|---|---|---|
对照Control | 13.6 ± 1.1 c | 3.5 ± 0.7 b | 232.6 ± 70.4 c |
添加葡萄糖Adding glucose | 19.3 ± 0.4 ab | 3.9 ± 0.1 a | 293.3 ± 56.5 b |
灭菌Sterilization | 16.3 ± 0.5 b | 3.4 ± 0.2 b | 295.3 ± 10.9 b |
灭菌后添加葡萄糖Sterilization and add glucose | 21.2 ± 1.1 a | 4.2 ± 0.3 a | 326.3 ± 36.4 a |
处理 Treatment | 株高/cm Plant height | 茎粗/mm Stem diameter | 叶长/cm Leaf length | 叶宽/cm Leaf width | 叶面积/cm2 Leaf area |
---|---|---|---|---|---|
对照Control | 38.67 ± 2.89 c | 3.86 ± 0.04 c | 8.06 ± 0.90 b | 4.83 ± 0.38 c | 25.21 ± 0.24 b |
添加葡萄糖Adding glucose | 45.00 ± 1.00 b | 4.65 ± 0.12 a | 8.61 ± 0.71 ab | 5.78 ± 0.44 a | 35.04 ± 0.28 a |
灭菌Sterilization | 40.04 ± 3.07 c | 4.14 ± 0.28 b | 8.15 ± 0.34 b | 5.10 ± 0.50 b | 27.11 ± 4.92 b |
灭菌后添加葡萄糖 Sterilization and add glucose | 49.00 ± 1.73 a | 4.59 ± 0.10 a | 8.90 ± 0.57 a | 5.85 ± 0.37 a | 38.14 ± 3.83 a |
表5 土壤添加葡萄糖和灭菌对苹果植株生长发育的影响
Table 5 Effects of adding glucose and soil sterilization on the growth and development of apple plants
处理 Treatment | 株高/cm Plant height | 茎粗/mm Stem diameter | 叶长/cm Leaf length | 叶宽/cm Leaf width | 叶面积/cm2 Leaf area |
---|---|---|---|---|---|
对照Control | 38.67 ± 2.89 c | 3.86 ± 0.04 c | 8.06 ± 0.90 b | 4.83 ± 0.38 c | 25.21 ± 0.24 b |
添加葡萄糖Adding glucose | 45.00 ± 1.00 b | 4.65 ± 0.12 a | 8.61 ± 0.71 ab | 5.78 ± 0.44 a | 35.04 ± 0.28 a |
灭菌Sterilization | 40.04 ± 3.07 c | 4.14 ± 0.28 b | 8.15 ± 0.34 b | 5.10 ± 0.50 b | 27.11 ± 4.92 b |
灭菌后添加葡萄糖 Sterilization and add glucose | 49.00 ± 1.73 a | 4.59 ± 0.10 a | 8.90 ± 0.57 a | 5.85 ± 0.37 a | 38.14 ± 3.83 a |
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