关键词: 葡萄, 果实发育, DNA甲基转移酶抑制剂, 5-氮杂胞苷, 可变剪接, 甲基化

Abstract:

Both DNA methylation and alternative splicing play important roles in fruit development，but the links and the interactions between them during fruit development are unclear. In this study，alternative splicing events occurring at different developmental stages of 5-azacytidine（5-azaC，DNA methyltransferase inhibitors）treated and control berries of Kyoho from 25 to 65 days after flowering were analyzed based on RNA-seq data. The results showed that alternative 3′ splice site（A3）was the most frequent type and mutually exclusive exons（MX）was the least type revealed in both the treatment and control groups. There was stage-specific regulation of alternative splicing during grape berry development. The events of alternative splicing were more abundant at the early stages of berry development，and 9 683 was identified as the conserved events during berry development. There were 671 alternative splicing events which showed significant deviation between the treatments and controls，and functional annotation demonstrated that 14 of these genes were associated with methylation modifications. The significant differences in the extent of alternative splicing of these methylation-related genes in the treated and control groups suggest that alternative splicing and methylation modification synergistically regulate the grape berry ripening process.

Key words: grape, fruit development, DNA methyltransferase inhibitor, 5-azaC, alternative splicing, methylation