苹果溶质转运蛋白基因MdSLC35F2-like表达与花青苷积累的研究

doi:10.16420/j.issn.0513-353x.2021-0764

园艺学报 ›› 2022, Vol. 49 ›› Issue (11): 2293-2303.doi: 10.16420/j.issn.0513-353x.2021-0764

• 研究论文 • 下一篇

苹果溶质转运蛋白基因MdSLC35F2-like表达与花青苷积累的研究

王智宇¹^,², 常贝贝¹^,², 刘琦¹^,², 程晓帆¹^,², 杜晓云¹, 于晓丽¹, 宋来庆¹^,^*(), 赵玲玲¹^,^*()

¹山东省烟台市农业科学研究院，烟台市苹果种质创新与品质调控重点实验室，山东烟台 265500
²烟台大学生命科学学院，山东烟台 264005

收稿日期:2022-06-06 修回日期:2022-07-15 出版日期:2022-11-25 发布日期:2022-11-25
通讯作者: 宋来庆,赵玲玲 E-mail:sdslq@126.com;zhaolingling122@163.com
基金资助:
国家现代农业产业技术体系专项资金项目(CARS-28);山东省重点研发项目(2018GHZ005);山东省农业良种工程项目(2021LZGC021);,山东省外专双百计划项目(WST2018013);烟台市科技发展计划项目(2020XCZX034)

Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple

WANG Zhiyu¹^,², CHANG Beibei¹^,², LIU Qi¹^,², CHENG Xiaofan¹^,², DU Xiaoyun¹, YU Xiaoli¹, SONG Laiqing¹^,^*(), ZHAO Lingling¹^,^*()

¹Key Laboratory of Apple Germplasm Innovation and Quality Control of Yantai，Yantai Academy of Agricultural Sciences，Yantai，Shandong 265500，China
²College of Life Sciences，Yantai University，Yantai，Shandong 264005，China

Received:2022-06-06 Revised:2022-07-15 Online:2022-11-25 Published:2022-11-25
Contact: SONG Laiqing,ZHAO Lingling E-mail:sdslq@126.com;zhaolingling122@163.com

摘要/Abstract

摘要：

以‘长富2’苹果片红芽变的果皮为试材，克隆了溶质转运蛋白家族基因MdSLC35F2-like（Solute Carrier Family 35 Member F2-like）的开放阅读框（ORF），其长度为1 041 bp，编码346个氨基酸。利用在线软件进行生物信息分析，该蛋白是1个稳定的疏水性α螺旋跨膜蛋白，定位于细胞膜。同源性分析表明，MdSLC35F2-like与白梨的PbSLC35F1-like同源性最高;系统进化分析表明，其与白梨的进化亲缘关系最近，与可可、巨桉的进化亲缘关系最远。分析‘长富2号’及其片红芽变果实着色期花青苷的积累与MdSLC35F2-like表达量的变化，结果表明，摘袋后MdSLC35F2-like表达量在‘长富2号’及其2个片红芽变果实果皮中均呈先升高后降低的趋势，在摘袋后3 d表达量最高，而后逐渐降低，2个芽变材料均显著高于其野生型，表达量与花青苷含量呈正相关关系。在其他颜色品种上进行验证分析，MdSLC35F2-like在红色果皮品种‘烟富3号’果皮中的表达量极显著高于绿色果皮品种‘金陵’，在红色果肉品种‘红色之爱’果肉中的表达量显著高于白肉品种‘烟农早富’。以上结果进一步证明MdSLC35F2-like与花青苷积累有关。

关键词: 苹果, 花青苷, MdSLC35F2-like, 表达分析

Abstract:

Anthocyanin is the main pigment affecting the skin color of apple fruit. In our previous study，MdSLC35F2-like was expressed at higher levels in the‘Nagafu 2’apple red bud mutant than in the wild-type. The open reading frame（ORF）of MdSLC35F2-like was cloned，it is 1 041bp，encoding 346 amino acids，which was predicted to be a hydrophobic α helix transmembrane protein with the highest homology to the PbSLC35F1-like of Pyrus × bretschneideri. Phylogenetic analysis showed that it had the closest evolutionary relationship with P. × bretschneideri，and the furthest evolutionary relationship with Theobroma cacao and Eucalyptus grandis. MdSLC35F2-like sequence and its expression rules before and after the fruit bagging were analyzed. After unbagging，the expression level of MdSLC35F2-like increased firstly，and then decreased with a peak on the third day. The expression level of MdSLC35F2-like in the two kinds of buds was significantly higher than that of‘Nagafu 2’. The above results indicated that the expression level of MdSLC35F2-like gene is positively correlated with anthocyanine content. Meanwhile，its expression levels in different color apple cultivars were compared. The expression of MdSLC35F2-like in red peel cultivars was significantly higher than that in green peel cultivars，and the expression level in red pulp cultivars was higher than that in non-red pulp cultivars. It suggests that MdSLC35F2-like may participate in the anthocyanine accumulation process.

Key words: apple, anthocyanin, MdSLC35F2-like expression analysis

中图分类号:

S661.1

王智宇, 常贝贝, 刘琦, 程晓帆, 杜晓云, 于晓丽, 宋来庆, 赵玲玲. 苹果溶质转运蛋白基因MdSLC35F2-like表达与花青苷积累的研究[J]. 园艺学报, 2022, 49(11): 2293-2303.

WANG Zhiyu, CHANG Beibei, LIU Qi, CHENG Xiaofan, DU Xiaoyun, YU Xiaoli, SONG Laiqing, ZHAO Lingling. Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple[J]. Acta Horticulturae Sinica, 2022, 49(11): 2293-2303.

图/表 9

图1 MdSLC35F2-like基因克隆

Fig. 1 Gene cloning of MdSLC35F2-like

图2 MdSLC35F2-like蛋白同源性比较 XP_0072029：毛桃;XP_0342249：扁桃;XP_0082412：红梅;XP_0218101：欧洲甜樱桃;CAB4287212：杏;XP_0093666：白梨;KAB2607766：秋子梨 × 西洋梨;XP_0289478：苹果。

Fig. 2 MdSLC35F2-like is compared by protein homology sequence XP_0072029：Prunus persica;XP_0342249：Prunus dulcis;XP_0082412：Prunus mume;XP_0218101：Prunus avium;CAB4287212：Prunus armeniaca;XP_0093666：Pyrus × bretschneideri;KAB2607766：Pyrus ussuriensis × Pyrus communis;XP_0289478：Malus × domestica.

图3 MdSLC35F2-like氨基酸同其他植物氨基酸序列的聚类分析

Fig. 3 Cluster analysis of MdSLC35F2-like amino acid and other plant amino acid sequences

图4 ‘长富2号’野生型及其片红芽变苹果摘袋后果皮着色变化

Fig. 4 Pigmentation changes of pericarp after unbagging in‘Nagafu 2’wild type and its slices of red buds

图5 ‘长富2号’野生型与及其片红芽变苹果果皮花青苷含量

Fig. 5 Content of anthocyanin in‘Nagafu 2’wild type and its slices of red buds * P < 0.05，** P < 0.01.

图6 ‘长富2号’母树野生型及其片红芽变苹果果皮中MdSLC35F2-like表达量分析

Fig. 6 Expression of MdSLC35F2-like in‘Nagafu 2’wild type and its slices of red buds * P < 0.05，** P < 0.01.

图7 花青苷含量与MdSLC35F2-like基因表达水平的相关性分析

Fig. 7 Correlation analysis between anthocyanin in content and MdSLC35F2-like expression level

图8 ‘金陵’（绿皮）和‘烟富3’（红皮）苹果果皮中花青苷含量与MdSLC35F2-like表达量分析

Fig. 8 Content of anthocyanin and expression of MdSLC35F2-like in‘Jinling’（green peel）and‘Yanfu 3’（red peel） ** P < 0.01.

图9 ‘烟农早富’（白肉）和‘红色之爱’（红肉）苹果果肉中花青苷含量与MdSLC35F2-like表达量分析

Fig. 9 Content of anthocyanin and expression of MdSLC35F2-like in‘Yannong Zaofu’（white pulp）and‘Red Love’（red pulp） * P < 0.05;** P < 0.01.

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苹果溶质转运蛋白基因MdSLC35F2-like表达与花青苷积累的研究

Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple

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苹果溶质转运蛋白基因MdSLC35F2-like表达与花青苷积累的研究

Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple

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