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园艺学报 ›› 2022, Vol. 49 ›› Issue (1): 100-116.doi: 10.16420/j.issn.0513-353x.2020-0850

• 研究论文 • 上一篇    下一篇

茶树CsTIFY家族全基因组鉴定及非生物胁迫和激素处理中主要基因表达分析

谢思艺1,3, 周承哲1,2,3, 朱晨1,2,3, 詹冬梅1,3, 陈兰1,3, 吴祖春1,3, 赖钟雄1,2, 郭玉琼1,3,*()   

  1. 1福建农林大学园艺学院,茶产业研究院,福州 350002
    2福建农林大学园艺植物生物工程研究所,福州 350002
    3福建农林大学茶学福建省高校重点实验室,福州 350002
  • 收稿日期:2021-04-23 修回日期:2021-07-26 出版日期:2022-01-25 发布日期:2022-01-24
  • 通讯作者: 郭玉琼 E-mail:guoyq828@163.com
  • 基金资助:
    福建农林大学“双一流”建设科技创新能力提升培育计划项目(KSYLP004);6.18协同创新院茶产业技术分院专项(K1520001A);福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A01);国家现代农业产业技术体系建设专项资金项目(CARS-19);福建农林大学科技创新专项基金项目(CXZX2017537);茶树种质资源生化分析与生物学研究专项(K1518023A)

Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis

XIE Siyi1,3, ZHOU Chengzhe1,2,3, ZHU Chen1,2,3, ZHAN Dongmei1,3, CHEN Lan1,3, WU Zuchun1,3, LAI Zhongxiong1,2, GUO Yuqiong1,3,*()   

  1. 1College of Horticulture,Tea Industry Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    2Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    3Key Laboratory of Tea Science of Fujian Province,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Received:2021-04-23 Revised:2021-07-26 Online:2022-01-25 Published:2022-01-24
  • Contact: GUO Yuqiong E-mail:guoyq828@163.com

摘要:

TIFY转录因子是陆地植物特有的转录因子,按照结构域特征共分为4个亚家族TIFY、PPD、JAZ、ZML,调控植物的生长发育过程。以茶树TIFY转录因子为研究对象,从茶树基因组数据库中共鉴定了22个CsTIFY转录因子家族成员,都包含了TIFY结构域。基因结构分析发现,大部分CsTIFY家族成员外显子数在5到12之间,同一亚家族所含Motif和外显子数相似。系统进化树表明,6个物种122个TIFY家族基因分为4个组,其中茶树与葡萄和杨树亲缘关系更近。启动子顺式作用元件分析发现CsTIFY家族含有大量响应SA、ABA、低温和干旱等的元件。亚细胞定位试验结果表明CsTIFY1、CsJAZ1和CsJAZ5定位于细胞核。不同茶树品种的组织表达量分析发现,大部分CsTIFY在成熟叶、根和果实中相对表达量较高。在外源ABA、SA和MeJA处理下,大部分CsJAZ上调表达,而CsZML的表达量下降;低温和干旱处理下,CsZML1表达量都极显著上升。

关键词: 茶树, TIFY转录因子, 全基因组鉴定, ABA, SA, MeJA, 非生物胁迫, 亚细胞定位

Abstract:

TIFY transcription factors are unique transcription factors of terrestrial plants. They are divided into four sub-families TIFY,PPD,JAZ,ZML according to structural domain characteristics,and regulate the growth and development of plants. In this paper,the TIFY transcription factor of tea plant is the research object. A total of 22 members of the CsTIFY transcription factor family have been identified from the tea plant genome database,all of which contain the TIFY domain. Gene structure analysis found that most of the CsTIFY family members have exons between five and 12,and the same subfamily contains similar numbers of motifs and exons. The results of the phylogenetic tree showed that the 122 genes of six species were divided into four groups,among which tea trees were more closely related to grapes and poplars. Analysis of promoter cis-acting elements found that the CsTIFY family contains a large number of phytohormones and abiotic stress response elements such as SA,ABA,low temperature and drought. The results of subcellular localization experiments showed that CsTIFY1,CsJAZ1 and CsJAZ5 were localized in the nucleus. The tissue expression analysis of different tea cultivars showed that most CsTIFY gene family members had higher relative expression levels in mature leaves,roots and fruits. In addition,the expression of most CsJAZ genes was increased and the expression of CsZML was decreased under the treatments of exogenous hormones ABA,SA and MeJA. The expression of CsZML1 was significantly increased under both low temperature and drought treatments.

Key words: Camellia sinensis, TIFY transcription factor, genome-wide identification, ABA, SA, MeJA, abiotic stress, subcellular localization

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