园艺学报 ›› 2021, Vol. 48 ›› Issue (3): 421-438.doi: 10.16420/j.issn.0513-353x.2020-0602

• 研究论文 • 上一篇    下一篇


贾兵, 郭国凌, 王友煜, 魏鹏飞, 余桃, 常笑, 衡伟()   

  1. 安徽农业大学园艺学院,合肥 230036
  • 收稿日期:2020-09-28 出版日期:2021-03-25 发布日期:2021-04-02
  • 通讯作者: 衡伟
  • 基金资助:

Relationship Between Morphogenesis and Carbohydrate Synthesis of the Calyx-Persistence Fruit and Calyx-shedding Fruit in Differentiation Stage of‘Dangshan Suli’Pear

JIA Bing, GUO Guoling, WANG Youyu, WEI Pengfei, YU Tao, CHANG Xiao, HENG Wei()   

  1. College of Horticulture,Anhui Agricultural University,Hefei 230036,China
  • Received:2020-09-28 Online:2021-03-25 Published:2021-04-02
  • Contact: HENG Wei


以‘砀山酥梨’为试材,于盛花期分别喷施清水、200 mg·L-1PP333、400 mg·L-1 GA3,在幼果分化期,分别取各处理的宿萼果和脱萼果,测定其单果质量、叶绿素、山梨醇、蔗糖、葡萄糖和果糖含量;运用荧光定量PCR技术,分析了与光合作用、山梨醇和蔗糖代谢相关基因的表达量及S6PDH、NAD+-SDH、NADP+-SDH、SPS和SUS酶活。结果表明,各处理宿萼果中山梨醇和蔗糖含量均显著高于脱萼果。宿萼果中显著增加的山梨醇和蔗糖含量,就幼果光合而言,与光系统Ⅱ蛋白亚基编码基因PSⅡpsbD、Rubisco活化酶基因Rubisco activaseⅣ 表达量显著高于脱萼果有关;从山梨醇合成途径来看,与S6PDH1表达量和山梨醇-6-磷酸脱氢酶活性显著高于脱萼果有关;从源–库的转运关系来看,与山梨醇转运蛋白基因SOT3/4/8/14/15/21/25/29/30/31/34表达量显著高于脱萼果有关;从蔗糖合成途径来看,与磷酸蔗糖合成酶基因SPS1/8、蔗糖合成酶基因SUS1/3/7/12/13/15/17表达量及其酶活显著高于脱萼果有关;从蔗糖转运途径来看,与蔗糖转运蛋白SUT1/2/3、液泡膜单糖转运蛋白TMT2/3/4基因相对表达量显著高于脱萼果有关。因此,‘砀山酥梨’宿萼果中较脱萼果中显著增强的山梨醇、蔗糖代谢是影响其形态建成的原因。

关键词: 梨, 宿萼果, 脱萼果, 光合作用, 山梨醇, 蔗糖


Pyrus bretschneideri‘Dangshan Suli’was used as the experiment material,sprayed with distilled water,200 mg·L-1 PP333 and 400 mg·L-1 GA3 at full bloom stage;the calyx-persistence fruit and calyx-shedding fruit were respectively collected at the young fruit differentiation stage;the single fruit weight,chlorophyll,sorbitol,sucrose,glucose and fructose content were measured and the expression of genes related to photosynthesis,sorbitol and sucrose metabolism was analyzed by quantitative real-time PCR(qRT-PCR). Besides,the enzyme activities of S6PDH,NAD+-SDH,NADP+-SDH,SPS and SUS were determined. The results showed that the contents of sorbitol and sucrose in calyx-persistence fruit were significantly higher than those in calyx-shedding fruit of all treatments. In terms of the autologous photosynthesis,the significantly increase sorbitol and sucrose content in all calyx-persistence fruit could be supported by the obvious higher relative expression of PSII protein subunits psbD and Rubisco activase genes in calyx-persistence fruit than those in calyx-shedding fruit. From the perspective of sorbitol synthesis pathway,the prominent higher sorbitol content in calyx-persistence fruit was related to the higher expression of S6PDH1 and the outstanding higher activity of sorbitol-6-phosphate dehydrogenase. From the perspective of ‘source-sink’transport,it was related to the distinct higher expression of sorbitol transporter genes SOT3/4/8/14/15/2/25/29/30/31/34 in calyx-persistence fruit than that in calyx-shedding fruit. Likewise,the apparent increased sucrose content in calyx-persistence fruit was related to the significant higher expression of sucrose phosphate synthase genes SPS1 and SPS8,sucrose synthase genes SUS1/3/12/13/15/17,and their clear higher enzyme activities from the perspective of sucrose synthesis pathway. When it comes to the perspective of sucrose transport pathway,it was related to distinct higher expression of the sucrose transport genes SUT1/2/3and the tonoplast monosaccharide transporter TMT2/3/4in calyx-persistence fruit. This experiment preliminary clarified that the morphogenesis of calyx-persistence fruit and calyx-shedding fruit is determined by the relative sorbitol and sucrose metabolism activities.

Key words: pear, calyx-persistence fruit, calyx-shedding fruit, photosynthesis, sorbitol, sucrose