https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2022, Vol. 49 ›› Issue (5): 1081-1091.doi: 10.16420/j.issn.0513-353x.2021-0355

• 研究论文 • 上一篇    下一篇

金边红苞凤梨叶色突变体色素鉴定及类胡萝卜素合成限速基因筛选

周徐子鑫, 杨威, 毛美琴, 薛彦斌, 马均()   

  1. 四川农业大学风景园林学院,成都 611130
  • 收稿日期:2021-06-15 修回日期:2021-09-07 出版日期:2022-05-25 发布日期:2022-05-25
  • 通讯作者: 马均 E-mail:junma365@hotmail.com
  • 基金资助:
    国家自然科学基金项目(31770743);国家自然科学基金项目(31971704)

Identification of Pigment Components and Key Genes in Carotenoid Pathway in Mutants of Chimeric Ananas comosus var. bracteatus

ZHOU Xuzixin, YANG Wei, MAO Meiqin, XUE Yanbin, MA Jun()   

  1. College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2021-06-15 Revised:2021-09-07 Online:2022-05-25 Published:2022-05-25
  • Contact: MA Jun E-mail:junma365@hotmail.com

摘要:

以金边红苞凤梨叶片边缘呈红色时期的嵌合体叶片和金黄色突变体叶片为材料,进行CIEL*a*b*分析、色素含量测定、组织细胞观察、类胡萝卜素LC-MS/MS检测和转录组测序联合对比分析,对金黄色突变叶片的表型成因进行初步探究。CIEL*a*b*分析表明,嵌合叶片中央绿色部分与边缘红色部分的颜色分别由绿、黄和红、黄颜色组成,而金黄色突变叶片为黄色中带有极微量绿色。金黄色突变叶片的颜色特征是由于其总叶绿素含量和Chl.a/b值极显著低于正常绿色部分、总花青素极显著低于边缘红色部分、类胡萝卜素和叶绿素b含量占比极显著上升形成的。金黄色突变叶片中除少量细胞含有黄绿色质体外,其他叶肉细胞为无色透明,其中共检测到12种类胡萝卜素物质,叶黄素占比高达79%,是主要呈色物质。转录—代谢联合分析表明金黄色突变叶片中将碳流转化为类胡萝卜素的八氢番茄红素合成酶基因PSY1的表达水平以及该酶下游产物八氢番茄红素、α-胡萝卜素和叶黄素等物质的含量均低于中央绿色部分,PSY1可能是金黄色突变叶片类胡萝卜素合成受到限制的关键基因。

关键词: 红苞凤梨, 金黄色突变体, 类胡萝卜素, 代谢组, 转录组

Abstract:

In order to explore how the phenotype of yellow mutants of chimeric Ananas comosus var. bracteatus formed,chimeral leaves in red stage and yellow mutants were taken in this study for comprehensive and comparative analysis using CIEL*a*b* coloration,pigment determination,histocytological observation,LC-MS/MS detection and illumina RNA-seq. Results of CIEL*a*b* indicated that the color of the green central part and red margin part of the chimeric leaves was consisted of green-yellow and red-yellow color,respectively. The yellow mutant showed bright yellow color with extremely slight green. The significant decrease of total chlorophyll content,Chl.a/b ratio,total anthocyanin content and the significant increase of the proportion of carotenoid and Chl.b of the yellow mutant leaf resulted in the loss of green and red color and turned to yellow. The yellow pigments were scattered in a bit of mesophyll cells of the yellow mutant leaf while the other leaf cells were transparent and colorless. Lutein accounted for 79% of the 12 detected carotenoids content which is the dominant pigment of the yellow mutant leaf. The decrease of PSY1 expression and phytoene,carotenoid,lutein content indicated that PSY1 probably was a key gene responsible for the carotenoid biosynthesis limitation in the yellow mutant of chimeric Ananas comosus var. bracteatus.

Key words: Ananas comosus var. bracteatus, yellow mutant, carotenoid, metabolome, transcriptome

中图分类号: