园艺学报 ›› 2021, Vol. 48 ›› Issue (2): 276-288.doi: 10.16420/j.issn.0513-353x.2020-0282

• 研究论文 • 上一篇    下一篇


张庆雯, 祁静静, 谢宇, 谢竹, 彭蕴, 李强, 彭爱红, 邹修平, 何永睿, 陈善春*(), 姚利晓*()   

  1. 西南大学/中国农业科学院柑桔研究所,国家柑桔品种改良中心,重庆 400712
  • 收稿日期:2020-06-24 修回日期:2020-09-07 出版日期:2021-02-25 发布日期:2021-03-09
  • 通讯作者: 陈善春,姚利晓;
  • 基金资助:

Preliminary Analysis of CsCalS5 and Callose Deposition in Citrus sinensis Infected with Candidatus Liberibacter asiaticus

ZHANG Qingwen, QI Jingjing, XIE Yu, XIE Zhu, PENG Yun, LI Qiang, PENG Aihong, ZOU Xiuping, HE Yongrui, CHEN Shanchun*(), YAO Lixiao*()   

  1. National Center for CitrusVarieties Improvement,Chongqing 400712,China
  • Received:2020-06-24 Revised:2020-09-07 Online:2021-02-25 Published:2021-03-09
  • Contact: CHEN Shanchun,YAO Lixiao;


胼胝质合成酶是控制胼胝质合成的关键酶,在植物生长发育和抗逆胁迫中具有重要作用。克隆并分析‘锦橙’胼胝质合成酶基因CsCalS5及其启动子序列,实时荧光定量PCR检测CsCalS5的组织表达模式以及植物生长调节剂、黄龙病菌和柑橘溃疡病菌的诱导表达模式,并通过组织切片观察感染黄龙病和柑橘溃疡病‘锦橙’叶脉胼胝质的沉积。结果显示,CsCalS5启动子序列中含有脱落酸和病原菌的响应元件;CsCalS5编码1 952个氨基酸含有保守结构域FKS1和β-1,3-葡聚糖合成酶功能域的跨膜蛋白;CsCalS5在‘锦橙’的茎中高表达,且受脱落酸的诱导;黄龙病菌侵染后叶脉韧皮部的胼胝质沉积明显,患病叶片CsCalS5的表达量是健康对照的4.02倍;柑橘溃疡病菌侵染叶片后,叶脉韧皮部胼胝质沉积无明显增加,且CsCalS5的表达量与健康对照无显著差异。综上,黄龙病菌可能通过上调‘锦橙’CsCalS5的表达促进韧皮部胼胝质的沉积,从而增强其防御能力,且这种抗性可能受到脱落酸的调控。

关键词: 柑橘, 胼胝质合成酶, CsCalS5, 表达分析, 黄龙病


Callose synthase is a key enzyme to control callose synthesis and plays an important role in plant growth and stress resistance. The CsCalS5 gene and promoter sequence of Citrus sinensis‘Jincheng’ were cloned and analyzed. Its tissue expression and induced expression with exogenous phytohormones,Candidatus Liberibacter asiaticus(CLas)and Xanthomonas citri subsp. Citri(Xcc)were detected by real-time fluorescent quantitative PCR. The callose deposition in vein of CLas-infected or Xcc-infected C. sinensis was observed by tissue section staining. The CsCalS5 promoter sequence contained abscisic acid (ABA)and pathogen response elements. The CsCalS5 gene encoded a transmembrane protein composed of 1 952 amino acids containing the conserved domains FKS1 and β-1,3-glucan synthetase functional domain. CsCalS5 was highly expressed in the stem of C. sinensis,and could be induced by ABA. The expression of CsCalS5 in CLas-infected leaves was 4.02 times of that in the healthy control,and the callose was obviously deposited in the phloem of vein of CLas-infected leaves. After Xcc infection,callose deposition was not significantly increased in the veins phloem,the expression of CsCalS5 was not significantly different from healthy controls. In conclusion,CLas might promote the deposition of callose in the phloem by up-regulating the expression of CsCalS5 in C. sinensis,which could be regulated by ABA.

Key words: Citrus sinensis, callose synthase, CsCalS5, expression analysis, Huanglongbing