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园艺学报 ›› 2023, Vol. 50 ›› Issue (1): 27-35.doi: 10.16420/j.issn.0513-353x.2021-0927

• 研究论文 • 上一篇    下一篇

山金柑四倍体资源的发掘与鉴定

蒋靖东1, 韦壮敏2, 王楠1, 朱晨桥1, 叶俊丽1, 谢宗周1,*(), 邓秀新1, 柴利军1,*()   

  1. 1华中农业大学园艺林学学院,果蔬园艺作物种质创新与利用全国重点实验室,武汉 430070
    2广西壮族自治区亚热带作物研究所,南宁530001
  • 收稿日期:2022-04-27 修回日期:2022-08-03 出版日期:2023-01-25 发布日期:2023-01-18
  • 通讯作者: *(E-mail:chailijun@mail.hzau.edu.cnxiezz@webmail.hzau.edu.cn
  • 基金资助:
    国家重点研发计划项目(2021YFD1200200);广东省重点领域研发计划项目(2022B0202070002);国家自然科学基金项目(32122075);国家自然科学基金项目(32072523);国家现代农业产业技术体系建设专项资金项目(CARS-27)

Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat(Fortunella hindsii

JIANG Jingdong1, WEI Zhuangmin2, WANG Nan1, ZHU Chenqiao1, YE Junli1, XIE Zongzhou1,*(), DENG Xiuxin1, CHAI Lijun1,*()   

  1. 1National Key for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,China
    2Guangxi Subtropical Crops Research Institute,Nanning 530001,China
  • Received:2022-04-27 Revised:2022-08-03 Online:2023-01-25 Published:2023-01-18
  • Contact: *(E-mail:chailijun@mail.hzau.edu.cnxiezz@webmail.hzau.edu.cn

摘要:

在山金柑自然群体中通过流式细胞术发掘到1株四倍体植株,对其叶片、花朵、花粉、果实、种子等性状进行鉴定,并利用该材料自交创制新的四倍体材料。鉴定结果表明:四倍体相对于二倍体,叶片更宽、更厚、叶形指数变小,叶片气孔密度变小、气孔器更大;四倍体的花直径、整花长度、花瓣长度、花丝长度、雌蕊长度、花粉粒均极显著大于二倍体,花粉外壁纹饰密度极显著小于二倍体;四倍体的果形指数变大,果皮厚度增大,色泽指数无显著差异;四倍体种子横径与二倍体无显著差异,纵径极显著大于二倍体;其种子胚类型为多胚。四倍体山金柑自交后代均为四倍体,可作为柑橘育种和基础研究的资源。

关键词: 柑橘, 流式细胞术, 多倍体创制, 鉴定

Abstract:

A tetraploid plant was discovered in the native population of Hongkong kumquat (Fortunella hindsii)by flow cytometry. The leaf,flower,pollen,fruit,seed,and other characters of this tetraploid Hongkong kumquat were identified with the diploid Hongkong kumquat as control,and new tetraploid material was created by self-pollination of this tetraploid Hongkong kumquat. The results showed that compared with diploid Hongkong kumquat,the leaves of tetraploid are wider and thicker,leaf shape index becomes smaller,the stomatal density of the leaves becomes smaller and the stomatal apparatus is larger. The flowering diameter,whole flower length,petal length,filament length,pistil length,and pollen grain size of tetraploid are significantly larger than diploid,and the flower outer wall decoration density is significantly less than diploid,the fruit shape index of the tetraploid is larger,the peel thickness increases and the color index is not significantly different,the transverse diameter of tetraploid seeds is significantly different from diploid,the longitudinal diameter is significantly larger than diploid,and itsseed embryo type is polyembryonic. The progenies of tetraploid Hongkong kumquat are tetraploid. All the inbred progenies of tetraploid Hongkong kumquat are tetraploid. In this study,tetraploid Hongkong kumquat was discovered for the first time,its morphological indexes were identified. A new tetraploid was established in utilizing this material,which provided valuable materials for future citrus breeding and basic research.

Key words: citrus, flow cytometry, polyploidy creation, identification

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