园艺学报 ›› 2023, Vol. 50 ›› Issue (1): 27-35.doi: 10.16420/j.issn.0513-353x.2021-0927

• 研究论文 • 上一篇    下一篇


蒋靖东1, 韦壮敏2, 王楠1, 朱晨桥1, 叶俊丽1, 谢宗周1,*(), 邓秀新1, 柴利军1,*()   

  1. 1华中农业大学园艺林学学院,果蔬园艺作物种质创新与利用全国重点实验室,武汉 430070
  • 收稿日期:2022-04-27 修回日期:2022-08-03 出版日期:2023-01-25 发布日期:2023-01-18
  • 通讯作者: *(
  • 基金资助:

Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat(Fortunella hindsii

JIANG Jingdong1, WEI Zhuangmin2, WANG Nan1, ZHU Chenqiao1, YE Junli1, XIE Zongzhou1,*(), DENG Xiuxin1, CHAI Lijun1,*()   

  1. 1National Key for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,China
    2Guangxi Subtropical Crops Research Institute,Nanning 530001,China
  • Received:2022-04-27 Revised:2022-08-03 Online:2023-01-25 Published:2023-01-18
  • Contact: *(



关键词: 柑橘, 流式细胞术, 多倍体创制, 鉴定


A tetraploid plant was discovered in the native population of Hongkong kumquat (Fortunella hindsii)by flow cytometry. The leaf,flower,pollen,fruit,seed,and other characters of this tetraploid Hongkong kumquat were identified with the diploid Hongkong kumquat as control,and new tetraploid material was created by self-pollination of this tetraploid Hongkong kumquat. The results showed that compared with diploid Hongkong kumquat,the leaves of tetraploid are wider and thicker,leaf shape index becomes smaller,the stomatal density of the leaves becomes smaller and the stomatal apparatus is larger. The flowering diameter,whole flower length,petal length,filament length,pistil length,and pollen grain size of tetraploid are significantly larger than diploid,and the flower outer wall decoration density is significantly less than diploid,the fruit shape index of the tetraploid is larger,the peel thickness increases and the color index is not significantly different,the transverse diameter of tetraploid seeds is significantly different from diploid,the longitudinal diameter is significantly larger than diploid,and itsseed embryo type is polyembryonic. The progenies of tetraploid Hongkong kumquat are tetraploid. All the inbred progenies of tetraploid Hongkong kumquat are tetraploid. In this study,tetraploid Hongkong kumquat was discovered for the first time,its morphological indexes were identified. A new tetraploid was established in utilizing this material,which provided valuable materials for future citrus breeding and basic research.

Key words: citrus, flow cytometry, polyploidy creation, identification