菜豆豆荚发育过程中内源激素与细胞壁代谢的关系

doi:10.16420/j.issn.0513-353x.2020-0388

园艺学报 ›› 2021, Vol. 48 ›› Issue (2): 289-299.doi: 10.16420/j.issn.0513-353x.2020-0388

菜豆豆荚发育过程中内源激素与细胞壁代谢的关系

谢国芳¹^,², 刘娜², 宋易², 管春花², 张明生¹^,^*()

¹贵州大学生命科学学院,农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳 550025
²贵阳学院食品与制药工程学院,贵州省果品加工工程技术研究中心,贵阳 550005

收稿日期:2020-07-01 修回日期:2020-11-03 出版日期:2021-02-25 发布日期:2021-03-09
通讯作者: 张明生 E-mail:mshzhang@163.com
基金资助:
国家自然科学基金项目(31601798);贵州省教育厅普通高等学校拔尖人才资助项目(黔教合KY字[2019]066)

The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development

XIE Guofang¹^,², LIU Na², SONG Yi², GUAN Chunhua², ZHANG Mingsheng¹^,^*()

¹Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region（Ministry of Education）,Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering（CICMEAB）,College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China
²Food and Pharmaceutical Engineering Institute/Guizhou Engineering Research Center for Fruit Processing,Guiyang University,Guiyang 550005,China

Received:2020-07-01 Revised:2020-11-03 Online:2021-02-25 Published:2021-03-09
Contact: ZHANG Mingsheng E-mail:mshzhang@163.com

摘要/Abstract

摘要：

细胞壁代谢严重影响鲜食菜豆（Phaseolus vulgaris L.）的食用品质。以‘大白棒’豆荚为试材,研究其4个发育阶段内源激素含量、细胞壁主要成分及酶活性的变化,并分析其内源激素与细胞壁代谢的关系。结果表明,豆荚发育过程中GA₃和6-BA含量呈下降趋势,乙烯（ET）生成速率和茉莉酸（JA）含量呈先降后增趋势;豆荚在花后前10 d以增长为主,花后10 d后以增粗为主;花后前10 d蔗糖合成酶（SuSy）促进蔗糖合成,随着SuSy活性和纤维素酶（Cx）活性的下降,蔗糖含量降低,纤维素含量升高;花后5 d时G木质素在木质部沉积,花后10 d时G木质素和S木质素均在韧皮部沉积,逐渐增多并向两边扩散,S木质素仅在韧皮部沉积;花后5 d时苯丙氨酸解氨酶（PAL）活性高,为木质素生物合成提供底物,随后肉桂醇脱氢酶（CAD）活性增加,促进木质素合成;超氧化物歧化酶（SOD）活性增加促进 $\mathrm{O}_{2}^{\overline{·}}$ 向H₂O₂转化,与增加的过氧化物酶（POD）催化木质素的氧化聚合;多聚半乳糖醛酸酶（PG）活性、共价性果胶（SSP）和水溶性果胶（WSP）含量呈现先增后降的趋势,果胶甲基酯酶（PME）活性呈下降趋势,离子型果胶（CSP）含量呈先降后增趋势。通过相关性分析发现,JA正向调控豆荚发育和纤维素代谢,ET、GA₃、6-BA、ABA则相反;JA通过抑制 $\mathrm{O}_{2}^{\overline{·}}$ 向H₂O₂转化和POD活性来调控木质素的聚合,ET、GA₃、6-BA、ABA通过增强PAL和POD活性来促进木质素合成;IAA和SA参与调控果胶代谢。

关键词: 菜豆, 豆荚发育, 内源激素, 细胞壁代谢

Abstract:

The cell wall metabolism of fresh common bean（Phaseolus vulgaris L.）seriously affects the edible quality. The contents of endogenous hormones,the main components and enzymes related to cell wall metabolism were analyzed in four developmental stages in beans‘Dabaibang’,and the relationship between endogenous hormones and cell wall metabolism was analyzed. The results showed that the content of GA₃ and 6-BA decreased,the content of ethylene（ET）and JA decreased firstly and then increased during the development of pods. The pods was mainly in elongation growth before the 10th day after anthesis,and the pods was mainly in thickening growth after the 10th day after anthesis. Sucrose synthetase（SuSy）promoted sucrose synthesis before 10 days after anthesis. Sucrose content decreased and cellulose content increased with the decrease of SuSy and cellulase（Cx）activity. G-lignin deposition in xylem at 5 days after anthesis,G-lignin and S-lignin were deposited in phloem,gradually deepened and diffused to both sides after the 10th day after anthersis. S-lignin was only deposited in phloem. The high phenylalanine ammonia lyase（PAL）activity provided the substrate for lignin biosynthesis at the 5th day after anthesis,and then the increase of cinnamyl alcohol dehydrogenase（CAD）activity promoted lignin synthesis,the increase of superoxide dismutase（SOD）activity promoted the conversion of $\mathrm{O}_{2}^{\overline{·}}$ to H₂O₂,and the polymerization of lignin with peroxidase（POD）activity. Polygalacturonase（PG）activity,sodium-carbonate-soluble-pectin（SSP）and water-soluble-pectin（WSP）content increased firstly and then decreased,pectin methylesterase（PME）activity decreased,chelator-soluble-pectin（CSP）content decreased firstly and then increased. The correlation analysis showed that JA positively regulated pod development and cellulose metabolism,while ET,GA₃,6-BA and ABA were the opposite. JA regulates lignin polymerization by inhibiting the conversion of $\mathrm{O}_{2}^{\overline{·}}$ to H₂O₂ and POD activity. ET,GA₃,6-BA and ABA promoted lignin synthesis by enhancing PAL and POD activity. IAA and SA were involved in regulation of pectin metabolism.

Key words: Phaseolus vulgaris, bean development, endogenous hormones, cell wall metabolism

中图分类号:

S643.1

谢国芳, 刘娜, 宋易, 管春花, 张明生. 菜豆豆荚发育过程中内源激素与细胞壁代谢的关系[J]. 园艺学报, 2021, 48(2): 289-299.

XIE Guofang, LIU Na, SONG Yi, GUAN Chunhua, ZHANG Mingsheng. The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development[J]. Acta Horticulturae Sinica, 2021, 48(2): 289-299.

图/表 7

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花后天数 Days after anthesis	乙烯释放速率/ （mg · kg^-1· h^-1） ET	ABA/ （μg · g^-1 FW）	GA₃/ （μg · g^-1 FW）	6-BA/ （μg · g^-1 FW）	JA/ （μg · g^-1 FW）	SA/ （μg · g^-1 FW）	IAA/ （μg · g^-1 FW）
5	86.22 ± 1.18 a	0.15 ± 0.00 a	1.42 ± 0.06 a	52.93 ± 1.39 a	1.22 ± 0.02 c	15.82 ± 0.33 b	0.09 ± 0.00 b
10	19.77 ± 0.41 b	0.12 ± 0.00 c	0.47 ± 0.02 b	7.15 ± 0.24 b	1.11 ± 0.00 c	10.98 ± 0.51 c	0.08 ± 0.00 b
15	16.97 ± 0.24 c	0.13 ± 0.00 b	0.30 ± 0.02 c	6.34 ± 0.26 b	2.14 ± 0.05 b	49.31 ± 0.73 a	0.88 ± 0.06 a
20	21.36 ± 0.82 b	0.11 ± 0.00 d	0.14 ± 0.01 d	0.51 ± 0.04 c	2.69 ± 0.04 a	8.50 ± 0.28 d	0.11 ± 0.01 b

花后天数 Days after anthesis	乙烯释放速率/ （mg · kg^-1· h^-1） ET	ABA/ （μg · g^-1 FW）	GA₃/ （μg · g^-1 FW）	6-BA/ （μg · g^-1 FW）	JA/ （μg · g^-1 FW）	SA/ （μg · g^-1 FW）	IAA/ （μg · g^-1 FW）
5	86.22 ± 1.18 a	0.15 ± 0.00 a	1.42 ± 0.06 a	52.93 ± 1.39 a	1.22 ± 0.02 c	15.82 ± 0.33 b	0.09 ± 0.00 b
10	19.77 ± 0.41 b	0.12 ± 0.00 c	0.47 ± 0.02 b	7.15 ± 0.24 b	1.11 ± 0.00 c	10.98 ± 0.51 c	0.08 ± 0.00 b
15	16.97 ± 0.24 c	0.13 ± 0.00 b	0.30 ± 0.02 c	6.34 ± 0.26 b	2.14 ± 0.05 b	49.31 ± 0.73 a	0.88 ± 0.06 a
20	21.36 ± 0.82 b	0.11 ± 0.00 d	0.14 ± 0.01 d	0.51 ± 0.04 c	2.69 ± 0.04 a	8.50 ± 0.28 d	0.11 ± 0.01 b

花后天数 Days after anthesis	豆荚长度/cm Pod length	单荚豆粒质量/g Seed weight per pod	豆荚厚度/mm Pod thickness	豆荚直径/mm Pod diameter	单荚质量/g Weight per pod	豆荚相对厚度/% Relative thickness of pod
5	11.90 ± 0.37 c	0 d	2.67 ± 0.02 c	5.34 ± 0.03 d	2.90 ± 0.12 d	100.00 ± 0.00 a
10	19.08 ± 0.09 b	0.70 ± 0.06 c	3.02 ± 0.04 b	9.79 ± 0.16 c	13.60 ± 0.32 c	61.65 ± 0.55 b
15	20.62 ± 0.25 b	1.23 ± 0.07 b	3.15 ± 0.10 b	11.28 ± 0.21 b	18.07 ± 0.47 b	55.86 ± 0.93 c
20	22.41 ± 0.84 a	1.83 ± 0.09 a	3.77 ± 0.07 a	13.76 ± 0.17 a	23.43 ± 1.10 a	54.77 ± 0.74 c

花后天数 Days after anthesis	豆荚长度/cm Pod length	单荚豆粒质量/g Seed weight per pod	豆荚厚度/mm Pod thickness	豆荚直径/mm Pod diameter	单荚质量/g Weight per pod	豆荚相对厚度/% Relative thickness of pod
5	11.90 ± 0.37 c	0 d	2.67 ± 0.02 c	5.34 ± 0.03 d	2.90 ± 0.12 d	100.00 ± 0.00 a
10	19.08 ± 0.09 b	0.70 ± 0.06 c	3.02 ± 0.04 b	9.79 ± 0.16 c	13.60 ± 0.32 c	61.65 ± 0.55 b
15	20.62 ± 0.25 b	1.23 ± 0.07 b	3.15 ± 0.10 b	11.28 ± 0.21 b	18.07 ± 0.47 b	55.86 ± 0.93 c
20	22.41 ± 0.84 a	1.83 ± 0.09 a	3.77 ± 0.07 a	13.76 ± 0.17 a	23.43 ± 1.10 a	54.77 ± 0.74 c

花后天数 Days after anthesis	蔗糖/% Sucrose	SuSy活性/（g · kg^-1 FW） SuSy activity	纤维素/（U · g^-1） Cellulose	Cx活性/（U · g^-1） Cx activity
5	2.24 ± 0.03 b	76.50 ± 2.74 a	349.89 ± 6.01 d	1.80 ± 0.08 a
10	2.71 ± 0.03 a	23.16 ± 1.07 b	523.22 ± 5.74 c	1.77 ± 0.00 a
15	2.06 ± 0.08 bc	20.16 ± 1.03 b	618.07 ± 4.26 a	1.24 ± 0.02 b
20	1.83 ± 0.06 c	20.78 ± 1.26 b	599.41 ± 5.83 b	0.48 ± 0.02 c

菜豆豆荚发育过程中内源激素与细胞壁代谢的关系

The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development

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花后天数/d Days after anthesis	总酚/（g · kg^-1 DW） Total phenols	PAL/（U · g^-1）	CAD/（U · g^-1）	$\mathrm{O}_{2}^{\overline{·}}$/ （mmol · min^-1 · kg^-1）	SOD/（U · g^-1）
5	1.21 ± 0.02 c	31.32 ± 1.77 a	47.29 ± 1.97 c	0.44 ± 0.00 a	509.60 ± 7.44 b
10	1.57 ± 0.05 b	24.32 ± 0.89 b	53.18 ± 2.26 c	0.31 ± 0.01 b	562.80 ± 48.52 b
15	1.41 ± 0.03 b	24.65 ± 1.67 b	70.73 ± 1.55 b	0.31 ± 0.01 b	904.80 ± 47.29 a
20	2.11 ± 0.06 a	33.95 ± 1.51 a	128.80 ± 11.59 a	0.28 ± 0.01 b	859.90 ± 43.25 a
花后天数/d Days after anthesis	H₂O₂/（mol · kg^-1）	POD/（U · g^-1）	G木质素/（μg · g^-1 DW） G-lignin	S木质素/（μg · g^-1 DW） S-lignin
5	1.74 ± 0.01 c	368.65 ± 12.52 a	104.65 ± 1.63 a	50.67 ± 1.10 a
10	2.69 ± 0.04 a	315.24 ± 9.71 b	86.27 ± 0.77 c	44.47 ± 0.29 c
15	2.22 ± 0.07 b	301.38 ± 13.97 b	91.63 ± 1.52 b	45.32 ± 0.31 bc
20	2.71 ± 0.03 a	230.33 ± 10.02 c	94.29 ± 0.72 b	48.77 ± 1.79 ab

花后天数/d Days after anthesis	PG /（U · g^-1）	PME /（U · g^-1）	CSP /（g · kg^-1 DW）	SSP /（g · kg^-1 DW）	WSP/（g · kg^-1 DW）
5	203.90 ± 9.08 b	50.74 ± 0.64 a	0.84 ± 0.01 a	0.79 ± 0.01 b	1.15 ± 0.05 b
10	193.40 ± 7.37 b	30.63 ± 0.41 c	0.78 ± 0.03 a	0.86 ± 0.02 b	1.34 ± 0.05 ab
15	445.00 ± 16.27 a	37.36 ± 3.36 b	0.34 ± 0.03 b	1.05 ± 0.04 a	1.42 ± 0.01 a
20	145.90 ± 5.40 c	33.60 ± 1.18 bc	0.84 ± 0.04 a	0.83 ± 0.02 b	0.88 ± 0.06 c

分类 Sorts	指标 Index		ET		GA₃		6-BA		IAA		ABA		SA		JA
发育性状 Developmental traits	豆荚长度 Pod length		-0.934^**		-0.984^**		-0.964^**		0.325		-0.824^**		0.146		0.716^**
	豆荚厚度 Pod thickness		-0.870^**		-0.958^**		-0.931^**		0.257		-0.875^**		0.076		0.822^**
	豆荚相对厚度 Relative thickness of pods		0.987^**		0.986^**		0.993^**		-0.392		0.779^**		-0.227		-0.614^*
	单荚质量 Weight per pods		-0.870^**		-0.955^**		-0.927^**		0.298		-0.857^**		0.116		0.827^**
	单荚豆粒质量 Seed weight per pods		-0.784^**		-0.903^**		-0.859^**		0.279		-0.838^**		0.097		0.896^**
纤维素代谢 Cellulose metabolism	蔗糖Sucrose		-0.694^*		-0.689^*		-0.643^*		0.868^**		-0.250		0.790^**		0.602^*
	蔗糖合成酶 SuSy		-0.306		-0.520		-0.456		-0.253		-0.765^**		-0.398		0.813^**
	纤维素酶 Cellulase		-0.901^**		-0.960^**		-0.928^**		0.496		-0.736^**		0.331		0.803^**
	纤维素Cellulose		-0.945^**		-0.969^**		-0.949^**		0.533		-0.728^**		0.376		0.720^**
木质素代谢 Lignin metabolism	总酚 Total phenols		0.885^**		0.909^**		0.944^**		-0.029		0.954^**		0.144		-0.595^*
	PAL		0.699^*		0.548		0.584^*		-0.507		0.177		-0.464		0.051
	CAD		-0.448		-0.649^*		-0.575		-0.045		-0.771^**		-0.195		0.888^**
	G木质素 G-lignin		0.876^**		0.773^**		0.816^**		-0.192		0.513		-0.105		-0.076
	S木质素 S-lignin		0.669^*		0.546		0.577^*		-0.369		0.194		-0.321		0.068
	SOD		0.172		0.324		0.256		0.075		0.399		0.207		-0.491
	$\mathrm{O}_{2}^{\overline{·}}$		-0.467		-0.652^*		-0.601^*		-0.176		-0.868^**		-0.359		0.802^**
	H₂O₂		0.494		0.620^*		0.614^*		0.472		0.912^**		0.620^*		-0.524
	POD		0.696^*		0.844^**		0.777^**		-0.042		0.803^**		0.115		-0.807^**
果胶代谢 Pectin metabolism	PME		0.894^**		0.850^**		0.892^**		-0.016		0.749^**		0.073		-0.312
	PG		-0.488		-0.318		-0.432		-0.287		-0.343		-0.312		-0.430
	CSP		0.408		0.335		0.305		-0.933^**		-0.173		-0.923^**		-0.228
	SSP		-0.556		-0.477		-0.462		0.907^**		-0.005		0.860^**		0.290
	WSP		-0.143		0.041		0.004		0.510		0.457		0.575		-0.444
分类 Sorts		指标 Index		ABA/IAA		GA₃/IAA		GA₃/ABA		ET/ABA		IAA/ET		GA₃/ET
发育性状 Developmental traits		豆荚长度 Pod length		-0.636^*		-0.960^**		-0.980^**		-0.891^**		-0.921^**		-0.293
		豆荚厚度 Pod thickness		-0.619^*		-0.933^**		-0.957^**		-0.813^**		-0.857^**		-0.456
		豆荚相对厚度 Relative thickness of pods		0.647^*		0.969^**		0.975^**		0.956^**		0.974^**		0.134
		单荚质量 Weight per pods		-0.655^*		-0.939^**		-0.955^**		-0.811^**		-0.855^**		-0.448
		单荚豆粒质量 Seed weight per pods		-0.657^*		-0.898^**		-0.911^**		-0.717^**		-0.767^**		-0.578^*
纤维素代谢 Cellulose metabolism		蔗糖Sucrose		-0.925^**		-0.710^**		-0.809^**		-0.726^**		-0.696^*		-0.070
		蔗糖合成酶 SuSy		-0.198		-0.532		-0.453		-0.207		-0.294		-0.864^**
		纤维素酶 Cellulase		-0.794^**		-0.965^**		-0.985^**		-0.867^**		-0.890^**		-0.342
		纤维素Cellulose		-0.781^**		-0.969^**		-0.991^**		-0.930^**		-0.941^**		-0.202
木质素代谢 Lignin metabolism		总酚 Total phenols		0.376		0.881^**		0.829^**		0.807^**		0.863^**		0.253
		PAL		0.415		0.528		0.564		0.762^**		0.713^**		-0.547
		CAD		-0.401		-0.666^*		-0.613^*		-0.360		-0.437		-0.823^**
		G木质素 G-lignin		0.270		0.747^**		0.698^*		0.899^**		0.880^**		-0.328
		S木质素 S-lignin		0.322		0.534		0.536		0.697^*		0.657^*		-0.422
		SOD		0.172		0.338		0.284		0.169		0.211		0.452
		$\mathrm{O}_{2}^{\overline{·}}$		-0.243		-0.653^*		-0.569		-0.398		-0.479		-0.709^**
		H₂O₂		-0.059		0.601^*		0.465		0.414		0.496		0.498
		POD		0.446		0.860^**		0.794^**		0.651^*		0.693^*		0.579^*
果胶代谢 Pectin metabolism		PME		0.257		0.819^**		0.747^**		0.864^**		0.884^**		-0.027
		PG		0.316		-0.261		-0.171		-0.495		-0.490		0.617^*
		CSP		0.815^**		0.356		0.505		0.480		0.417		-0.290
		SSP		-0.812^**		-0.490		-0.623^*		-0.614^*		-0.559		0.223
		WSP		-0.184		0.038		-0.041		-0.204		-0.117		0.675^*

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菜豆豆荚发育过程中内源激素与细胞壁代谢的关系

The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development

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