关键词: 葡萄, 转录组, 葡萄糖, 试管苗

Abstract: The effects of glucose metabolism and photosynthesis on‘Red Globe’grape plantlets were studied under different concentrations（0，1%，2% and 4%）of exogenous glucose by the transcriptome sequencing. The result showed that the growth status of grape plantlets was the best under 2% glucose treatment，the second was 4% and 1%，the control（0）was the weakest in all treatments. 21.53 Gb Clean Data was obtained by sequencing in 4 different concentrations of glucose，and the base ratio of Q30 was more than 89.49%. Compared with reference genome，the sequence alignment efficiency was between 68.31% and 73.71% in all samples. The transfer efficiency of Single Nucleotide Polymorphisms（SNPs）ranged from 64.73% to 66.76%，and transversion was in 33.24%–35.27%. The most frequent types of mutations were A?G and C?T. The main event that occured in alternative splicing type was that the first and last exon were spliced. By setting a threshold to screening differentially express genes（DEGs），a total of 3 272 DEGs were obtained，including 2 826 down-regulated genes and 446 up-regulated genes. In the COG annotation classification，1%，2% and 4% were compared to the control，and annoted to 1 449，846 and 597 DEGs respectively，most of them are transcriptionally related genes except for general functional prediction. By detecting enzymes related leaf-carbon metabolism，SS and G6PDH activities were decreased first，then increased when the glucose concentration was added more. SS and G6PDH activities reached their peak value at 4% and 0，respectively. NI and SPS had same trend and both of their activity peak approached under the concentration of 2%. There was a consensus between expressional trend and sequenceing of 12 genes among 14 genes，indicating different concentrations of glucose regulate the expression of genes related leaf-carbon metabolism to improve the growth of‘Red Globe’.

Key words: grape, transcriptome, glucose, plantlet