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园艺学报 ›› 2018, Vol. 45 ›› Issue (1): 71-84.doi: 10.16420/j.issn.0513-353x.2017-0480

• 研究论文 • 上一篇    下一篇

结球甘蓝自花和异花授粉诱导的柱头转录组分析

蒲 敏1,廉小平2,*,罗绍兰1,张贺翠1,王玉奎1,白晓璟1,曾 静1,高启国2,任雪松2,朱利泉1,**   

  1. 1西南大学农学与生物科技学院,重庆 400700;2重庆市蔬菜重点实验室,重庆 400700
  • 出版日期:2018-01-25 发布日期:2018-01-25

Transcriptome Analysis of Stigma After Pollination in Brassica oleracea var. capitata

PU Min1,LIAN Xiaoping2,*,LUO Shaolan1,ZHANG Hecui1,WANG Yukui1,BAI Xiaojing1,ZENG Jing1,GAO Qiguo2,REN Xuesong2,and ZHU Liquan1,**   

  1. 1College of Agronomy and Biotechnology,Southwest University,Chongqing 400700,China;2 Key Laboratory in Olericulture of Chongqing,Chongqing 400700,China
  • Online:2018-01-25 Published:2018-01-25

摘要: 分别以自花授粉30 min、异花授粉30 min和未授粉处理的甘蓝柱头进行Illumina HiSeq2000高通量转录组测序,分析自交不亲和性甘蓝自花授粉、异花授粉柱头与未授粉柱头中基因表达的差异。转录组测序共获得1.6 × 108对原始序列读取片段,总碱基数为2.38 × 1010 bp,与未授粉对照相比,自花、异花授粉后差异表达的基因分别有2 900个和2 328个,共有的差异表达基因1 904个。在差异表达基因背景和全部基因背景下,自花授粉较大比例的差异基因是细胞杀伤、胞外基质部分和核酸结合转录因子活性;异花授粉较大比例的差异基因是信号传递、胞外区域部分、结构分子活性和营养库活性。GO功能的显著性富集分析表明,自花授粉处理后特有的40个极显著的富集条目主要涉及糖跨膜转运活性、微管结合和钙调素依赖性蛋白激酶活性等。异花授粉特有的53个极显著的富集条目主要涉及法尼酸O–甲基转移酶的活性、微管负向运动和生长素跨膜运输等。自花授粉处理后特异差异表达的996个基因主要涉及钙离子结合、细胞骨架相关、茉莉酸和水杨酸代谢等生物过程,异花授粉处理后特异差异表达的424个基因主要涉及物质跨膜转运及脂质代谢。

关键词: 结球甘蓝, 自交不亲和性, 转录组, 差异表达基因

Abstract: Illumina HiSeq2000,a high-through transcriptome sequencing technology,was applied to obtain the transcriptome differential expression data of stigma after self-pollinated 30 min,cross-pollinated 30 min and unpollinated. The objective of this study is to analyze the differences of gene expression of stigma after self-pollination and cross-pollination in Brassica oleracea L. var. capitata,and further analyze the significantly different expression genes. Transcriptome sequencing obtains a total of 2.38 × 1010 bp bases containing 1.6 × 108 of the original pair-end reads. There were 2 900 and 2 328 genes with altered expression in stigma after self-pollination and cross-pollination in Brassica oleracea L. var. capitata,respectively,and a total of 1 904 common genes expressed between two samples. The greater proportional difference after self-pollination is cell killing,extracellular matrix part and nucleic acid binding transcription factor activity. The greater proportional difference after cross-pollination is the signaling,extracellular region part,nutrient reservoir activity and structural molecule activity in the background of differential expression genes and all genes. GO enrichment analysis illustrated that the 40 specific highly significant GO terms in self-pollination sample mainly involved in sugar transmembrane transporter activity,tubulin binding and calmodulin-dependent protein kinase activity. The 53 specific highly significant GO terms in cross-pollination sample mainly involved in farnesoic acid O-methyltransferase activity,minus-end-directed microtubule motor activity and auxin influx transmembrane transporter activity. About 563 and 453 genes their expression was significantly up-regulated and down-regulated respectively in the 996 specific differentially expressed genes in self-pollination sample,which mainly related to calciumion binding,cytoskeletal correlation,jasmonic acid mediated signalin0g pathway and salicylic acid mediated signaling pathway. The 424 specific differentially expressed genes in cross-pollination sample mainly involved in transmembrane transport of substance and lipid metabolism.

Key words: Brassica oleracea L.var. capitata, self-incompatibility, transcriptome, differentially expressed gene