关键词: 甘蓝, 远缘杂交, 亲和指数, 基因传递效率, 遗传回复率

Abstract: In order to determine whether the seed-setting of distant hybridization between Brassica napus and B. oleracea is affected by different B. oleracea sub-species or not，in this study，two Chinese kale and four cabbage materials with Ogura CMS cytoplasm were crossed with two homozygous rapeseed restored lines harboring the Ogura CMS fertility-restored gene Rfo. The F1 interspecific hybrid plants with doubled chromosome were backcrossed with Ogura CMS Chinese kale and cabbage to produce BC1 plants，respectively. The seed-setting and the transmission rate of Rfo of Chinese kale × rapeseed cross combinations（CKR）and cabbage × rapeseed cross combinations（CR）were investigated in two generations，respectively. The results suggested that the compatibility index of CKR was two folds higher than that of CR，with significant difference between CKR and CR in two generations（P < 0.05）. In addition，the Rfo gene transmission rate of CKR was higher than that of CR. The pollen viability and recovering ratio of genetic background（RRGB）of all BC1 Rfo-positive individuals obtained from CKR and CR were assessed. The pollen viability of most BC1 Rfo-positive individuals obtained from CKR were significantly higher than that of the two Rfo-positive individuals obtained from CR（P < 0.05）. The RRGB of 26 Rfo-positive individuals obtained from CKR were 0.50–0.84. While the RRGB of two Rfo-positive individuals（15Y1 and 15Y4）obtained from CR were 0.53 and 0.59，respectively. Our results revealed that compared with cabbage，Chinese kale is much easier to produce progenies when crossed with B. napus and the transmission rate of alien fragment was much higher. Therefore，Chinese kale could be used as a bridge to accelerate the process of the beneficial agronomic traits transfer from B. napus to cabbage.

Key words: Brassica oleracea, distant hybridization, compatibility index, transmission rate of Rfo, recovering ratio of genetic background