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园艺学报 ›› 2022, Vol. 49 ›› Issue (1): 62-72.doi: 10.16420/j.issn.0513-353x.2020-1058

• 研究论文 • 上一篇    下一篇

大白菜花茎蜡粉近等基因系转录组分析

王荣花, 王树彬, 刘栓桃, 李巧云, 张志刚, 王立华, 赵智中()   

  1. 山东省农业科学院蔬菜花卉研究所,国家蔬菜改良中心山东分中心,山东省设施蔬菜生物学重点实验室,农业农村部黄淮地区蔬菜科学观测实验站(山东),济南250100
  • 收稿日期:2021-03-01 修回日期:2021-08-19 出版日期:2022-01-25 发布日期:2022-01-24
  • 基金资助:
    山东省自然科学基金项目(ZR2019BC048);山东省农业良种工程项目(2019LZGC006);山东省农业重大应用技术创新项目(2018);山东省农业科学院农业科技创新工程项目(CXGC2018E08)

Transcriptome Analysis of Waxy Near-isogenic Lines in Chinese Cabbage Floral Axis

WANG Ronghua, WANG Shubin, LIU Shuantao, LI Qiaoyun, ZHANG Zhigang, WANG Lihua, ZHAO Zhizhong()   

  1. Vegetable and Flower Institute,Shandong Academy of Agricultural Sciences,Shandong Branch of National Center for Vegetable Improvement,Shandong Key Laboratory for Biology of Greenhouse Vegetables,Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables,Ministry of Agriculture and Rural Affairs(Shandong),Ji’nan 250100,China
  • Received:2021-03-01 Revised:2021-08-19 Online:2022-01-25 Published:2022-01-24

摘要:

为研究大白菜花茎蜡粉分子调控机制,针对大白菜一对花茎蜡粉差异的近等基因系进行了转录组比较分析。在有蜡粉材料RHL065_1和无蜡粉材料RHL065_2之间总共鉴定到7 237个基因存在显著差异表达,主要富集在碳水化合物生物过程、原生质膜的组成成分和四吡咯结合分子功能。结合基因功能注释,筛选到17个蜡粉相关基因,包括蜡粉合成基因MAH1CYTB5-B(Bra022898,Bra021809)、KCS6/CER6/CUT1KCS9、FAR3/CER4、KCS1CER2、KCD/PAS2、CYTB5-C(Bra039268,Bra004518)、CER2-LIKE,蜡粉转运基因LTPG1(Bra010912,Bra030067)和转录因子基因WIN1/SHINE1MYB30DEWAX在两自交系间存在显著差异表达,其可能参与了大白菜花茎表皮蜡粉形成。利用qRT-PCR验证了转录组分析的可靠性以及差异表达基因在不同组织的表达模式。研究结果有助于进一步克隆大白菜蜡粉调控基因和开发相关功能分子标记。

关键词: 大白菜, 蜡粉, 转录组, 候选基因

Abstract:

In order to study the molecular mechanism of floral axis waxiness in Chinese cabbage,a comparative transcriptome analysis was carried out on a pair of near isogenic lines with difference in floral axis waxiness. In total,7 237 differentially expressed genes(DEGs)were identified between waxy material (RHL065_1)and non-waxy material(RHL065_2),mainly enriched in carbohydrate biological processes,cellular components of plasma membrane and tetrapyrrole binding molecular functions. Combined with gene function annotation,17 DEGs,involved in wax synthesis MAH1,CYTB5-B (Bra022898,Bra021809),KCS6/CER6/CUT1,KCS9,FAR3/CER4,KCS1,CER2,KCD/PAS2,CYTB5-C(Bra039268,Bra004518),CER2-LIKE,wax transport LTPG1(Bra010912,Bra030067)and transcription factors WIN1/SHINE1,MYB30,DEWAX were proposed to contribute to surface waxiness formation in Chinese cabbage floral axis. A further qRT-PCR analysis was used to verify the transcriptome analysis and survey the expression pattern of DEGs. These results will be helpful for further gene cloning,developing functional molecular markers and understanding the genetic architecture for waxiness.

Key words: Chinese cabbage, waxy, transcriptome, candidate gene

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