关键词: 苹果, 脱乙酰化酶, MdHDA19, 盐胁迫, 温度, 原核表达

Abstract:

Histone Deacetylases gene MdHDA19（MDP0000132078）was cloned from‘Gala’apple. Its ORF was 1 494 bp，which encoded 497 amino acids. The molecular mass of this protein was 55.73 kD，and pI was 4.98. The phylogenetic tree indicated that the HDA19 had sequence conservation among different species. MdHDA19 exhibited the highest sequence similarity with Theobroma cacao TcHDA19 （87.05%）. The MdHDA19 gene was expressed in all the tissues of apple，especially in root and flower. The MdHDA19 interacted with MdSAT18. The NaCl stress induces the expression level of MdHDA19. MdHDA19 might involve NaCl stress response. The resulted showed that MdHDA19 gene was induced in low temperature（4 ℃）and high temperature（40 ℃）. In addition，the MdHDA19 recombinant protein was obtained. The results established the foundation for the further functions research of MdHDA19 protein.

Key words: apple, histone deacetylases, MdHDA19, NaCl stress response, temperature, prokaryotic expression