苹果茎痘病毒RT-PCR检测及分子变异分析

园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 9-16.

苹果茎痘病毒RT-PCR检测及分子变异分析

李丽丽1; 董雅凤2*;张尊平2;张志宏1;范旭东2;裴光前2

(¹ 沈阳农业大学园艺学院, 沈阳110161; ² 中国农业科学院果树研究所, 辽宁兴城125100)

收稿日期:2009-09-07 修回日期:2009-11-11 出版日期:2010-01-25 发布日期:2010-01-25
通讯作者: 董雅凤

RT-PCR Detection and Molecular Variability of Apple stem pitting virus

LI Li-li1;DONG Ya-feng2*;ZHANG Zun-ping2;ZHANG Zhi-hong1;FAN Xu-dong2;PEI Guang-qian2

(¹College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; ²Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China )

Received:2009-09-07 Revised:2009-11-11 Online:2010-01-25 Published:2010-01-25
Contact: DONG Ya-feng

摘要/Abstract

摘要： 利用RT-PCR技术对采自6个苹果品种和7个梨品种的33个样本中的苹果茎痘病毒(Apple stem pitting virus, ASPV) 进行了检测, ASPV感染率为63% ( 19 /33) 。对6个ASPV中国分离物外壳蛋白(Coat protein, CP) 基因进行了扩增、克隆和测序, 测序结果显示CP基因全长为1 191 nt, 编码397个氨基酸。中国分离物与GenBank中其他分离物核苷酸序列同源性为70.2% ～91.7%。系统进化分析显示不同ASPV分离物分为3个大的类群, 呈现一定的寄主相关性, 并未呈现明显的地理相关性。第一和第三大类群的寄主皆为苹果, 第二大类群包含6个梨分离物和2个苹果分离物。不同ASPV分离物的抗原指数差异较大, 推断可能由于序列变异引起抗原表位变异, 从而引起血清型差异。

关键词: 苹果, 梨, 苹果茎痘病毒, RT-PCR检测, 序列分析

Abstract: Thirty-three samples including 6 apple and 7 pear cultivars were detected for Apple stem pitting virus (ASPV) by RT-PCR, the infected rate was up to 63% (19 /33). The ASPV coat protein (CP) gene of 6 Chinese isolates were amplified by RT-PCR, cloned and sequenced. Sequence analysis showed that the full-length CP gene comprised 1 191 nt and encoded 397 amino acid. Identities of nucleotide between Chinese isolates and other isolates were 70.2% - 91.7%. Phylogenetic analysis showed that all isolates of ASPV fell into three groups, and the group swere slightly related to host, but it was not related to geographical origin of isolates. In group 1 and 3, the host of all isolates were apples. Six isolates came from pears and two isolates came from apples lay in group 2. The diversity of antigenic index was complicated for different ASPV isolates, and we presumed that the diversity of antigenic index was due to variation of sequences, so serology differentiations were likely to be gradually developed.

Key words: apple, pear, Apple stem pitting virus, RT-PCR detection, sequence analysis

中图分类号:

S661
S718.85

李丽丽;董雅凤;张尊平;张志宏;范旭东;裴光前. 苹果茎痘病毒RT-PCR检测及分子变异分析[J]. 园艺学报, 2010, 37(1): 9-16.

LI Li-li;DONG Ya-feng;ZHANG Zun-ping;ZHANG Zhi-hong;FAN Xu-dong;PEI Guang-qian. RT-PCR Detection and Molecular Variability of Apple stem pitting virus[J]. ACTA HORTICULTURAE SINICA, 2010, 37(1): 9-16.

[1]	于婷婷, 李欢, 宁源生, 宋建飞, 彭璐琳, 贾竣淇, 张玮玮, 杨洪强. 苹果GRAS全基因组鉴定及其对生长素的响应分析[J]. 园艺学报, 2023, 50(2): 397-409.
[2]	韩晓蕾, 张彩霞, 刘锴, 杨安, 严家帝, 李武兴, 康立群, 丛佩华. 中熟苹果新品种‘中苹优蕾’[J]. 园艺学报, 2022, 49(S2): 1-2.
[3]	宋健坤, 杨英杰, 李鼎立, 马春晖, 王彩虹, 王然. 梨新品种‘鲁秀’[J]. 园艺学报, 2022, 49(S2): 3-4.
[4]	董星光, 曹玉芬, 张莹, 田路明, 霍宏亮, 齐丹, 徐家玉, 刘超, 王立东. 抗寒脆肉梨新品种‘玉翠香’[J]. 园艺学报, 2022, 49(S2): 5-6.
[5]	欧春青, 姜淑苓, 王斐, 马力, 张艳杰, 刘振杰. 早熟梨新品种‘兴梨蜜水’[J]. 园艺学报, 2022, 49(S2): 7-8.
[6]	张艳杰, 王斐, 欧春青, 马力, 姜淑苓, 刘振杰. 梨新品种‘中梨玉脆3’[J]. 园艺学报, 2022, 49(S2): 9-10.
[7]	范净, 陈启亮, 张靖国, 杨晓平, 杜威, 田瑞, 周德平, 胡红菊, . 中熟红皮砂梨新品种‘金彤’[J]. 园艺学报, 2022, 49(S2): 11-12.
[8]	王苏珂, 李秀根, 杨健, 王龙, 苏艳丽, 张向展, 薛华柏. 红皮梨新品种‘丹霞红’[J]. 园艺学报, 2022, 49(S2): 13-14.
[9]	韩晓蕾, 张彩霞, 刘锴, 严家帝, 李武兴, 康立群, 丛佩华. 中熟苹果新品种‘苹优2号’[J]. 园艺学报, 2022, 49(S1): 1-2.
[10]	王强, 丛佩华, 刘肖烽. 晚熟苹果新品种‘华优甜娃’[J]. 园艺学报, 2022, 49(S1): 3-4.
[11]	王强, 丛佩华, 刘肖烽. 中熟苹果新品种‘华优宝蜜’[J]. 园艺学报, 2022, 49(S1): 5-6.
[12]	杨玲, 丛佩华, 王强, 李武兴, 康立群. 中熟鲜食苹果新品种‘华丰’[J]. 园艺学报, 2022, 49(S1): 7-8.
[13]	王斐, 欧春青, 张艳杰, 马力, 姜淑苓. 晚熟耐贮梨新品种‘华秋’[J]. 园艺学报, 2022, 49(S1): 9-10.
[14]	宋健坤, 李鼎立, 杨英杰, 马春晖, 王彩虹, 王然. 梨新品种‘琴岛红’[J]. 园艺学报, 2022, 49(S1): 11-12.
[15]	丁志杰, 包金波, 柔鲜古丽, 朱甜甜, 李雪丽, 苗浩宇, 田新民. 新疆野苹果与‘元帅’‘金冠’的叶绿体基因组比对研究[J]. 园艺学报, 2022, 49(9): 1977-1990.