Classification and Expression Analysis of Apple MdCBL Family Genes with a Functional Characterization of MdCBL1

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (6): 1053-1062.

Classification and Expression Analysis of Apple MdCBL Family Genes with a Functional Characterization of MdCBL1

MA Qi-jun，HU Da-gang，YOU Chun-xiang，and HAO Yu-jin*

（State Key Laboratory of Crop Biology，MOA Key Laboratory of Horticultural Crop Biology（Huanghuai Region）and Germplasm Innovation，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China）

Received:2013-12-25 Online:2014-06-25 Published:2014-06-25

Abstract

Abstract: In this study，the functional domains of apple MdCBL family proteins were predicted with bioinformatics methods. Their phylogenetic relationship with 10 Arabidopsis AtCBL proteins were analyzed. They were systematically named based on the similarity to their Arabidopsis counterparts，respectively. Subsequently，Real-time quantitative RT-PCRs were performed to determine the expression levels of apple MdCBLs in different tissues and in response to various abiotic stresses including salt，low temperature，ABA and drought. Results demonstrated that MdCBLs may play roles in different tissues and be involved in the response to abiotic stresses in plants. Finally，MdCBL1 was genetically transformed into apple callus. Salt-tolerance assay indicated that overexpression of MdCBL1 remarkably increased the tolerance of transgenic apple callus to high salinity，further supporting that MdCBLs genes participate in the response of plants to abiotic stress.

Key words: apple, MdCBLs genes, abiotic stress, expression analysis, MdCBL1, callus

CLC Number:

S 661.1

MA Qi-jun，HU Da-gang，YOU Chun-xiang，and HAO Yu-jin*. Classification and Expression Analysis of Apple MdCBL Family Genes with a Functional Characterization of MdCBL1[J]. ACTA HORTICULTURAE SINICA, 2014, 41(6): 1053-1062.

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Classification and Expression Analysis of Apple MdCBL Family Genes with a Functional Characterization of MdCBL1

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