Proteomics Studies on the Early Stages of Botrytis cinerea Infection in Grapevine Leaves

doi:10.16420/j.issn.0513-353x.2024-0264

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (2): 279-291.doi: 10.16420/j.issn.0513-353x.2024-0264

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Proteomics Studies on the Early Stages of Botrytis cinerea Infection in Grapevine Leaves

ZHAO Yulei, LI Shan, LI Chengnan, MA Jinlong, MA Hongyi, YIN Xiao^*()

College of Enology and Horticulture，Ningxia University，Yinchuan 750021，China

Received:2024-11-13 Revised:2024-12-16 Online:2025-02-25 Published:2025-02-23
Contact: YIN Xiao

Abstract

Abstract:

Inoculating young leaves of susceptible Vitis vinifera‘Pinot Noir’and highly resistant V. amurensis‘Shuangyou’with Botrytis cinerea and treating them with sterile water as the control，label-free quantitative proteomics technology were used to analyze the differentially expressed proteins in the initial stage of B. cinerea infection（Inoculation for 12 h）. A total of 12 210 proteins were identified from the two grapevine cultivars. In‘Pinot Noir’，868 differentially expressed proteins were detected，while 1 946 differentially expressed proteins were detected in‘Shuangyou’. Through Gene Ontology（GO）and Kyoto Encyclopedia of Genes and Genomes（KEGG）enrichment analyses，it was determined that differentially expressed proteins were primarily enriched in metabolic pathways，secondary metabolite biosynthesis，and amino acid metabolism，among other signaling pathways. A total of 10 key differently expressed proteins were screened from the two grapevine varieties，and the expression levels of peroxidase，glutathione transferase，lipoxygenase，aldehyde dehydrogenase and stilbene synthase were up-regulated，while the expression levels of NADH dehydrogenase and carbonic anhydrase were down-regulated in highly resistant V. amurensis‘Shuangyou’. The above proteins play an important role in the infection of ‘Shuangyou’leaves by B. cinerea. Peroxidase was also expressed in the susceptible V. vinifera‘Pinot Noir’，indicating that peroxidase was an important differently expressed protein in the early stage of B. cinerea infection in grapevine leaves.

Key words: grapevine, Botrytis cinerea, proteomics, differentially expressed protein, quantitative Real-time PCR

ZHAO Yulei, LI Shan, LI Chengnan, MA Jinlong, MA Hongyi, YIN Xiao. Proteomics Studies on the Early Stages of Botrytis cinerea Infection in Grapevine Leaves[J]. Acta Horticulturae Sinica, 2025, 52(2): 279-291.

Figures/Tables 8

References 37

[1]	Agudelo-Romero P, Erban A, Rego C, Carbonell-Bejerano P, Nascimento T, Sousa L, Martínez-Zapater J M, Kopka J, Fortes A M. 2015. Transcriptome and metabolome reprogramming in Vitis vinifera cv. Trincadeira berries upon infection with Botrytis cinerea. Journal of Experimental Botany, 66 (7):1769-1785. doi: 10.1093/jxb/eru517 pmid: 25675955
[2]	Ahmad A, Akram W, Wang R, Shahzadi I, Umer M, Yasin N A, Wu T. 2022. Pathogenicity factors of Phytophthora melonis revealed by comparative proteomics. Journal of Plant Interactions, 17 (1):183-197.
[3]	Cabassa-Hourton C, Schertl P, Bordenave-Jacquemin M, Saadallah K, Guivarc'H A, Lebreton S, Planchais S, Klodmann J, Eubel H, Crilat E, Vos D L, Ghelis T, Richard L, Abdelly C, Carol P, Braun H, Savouré A. 2016. Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana. Biochemical Journal, 17 (473):2623-2634.
[4]	Chai Shengyue. 2021. Study on the function and mechanism of the transcription factor VaERF 99 for Botrytis cinerea resistance in Vitis amurensis[Ph. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	柴生樾. 2021. 中国野生山葡萄抗灰霉病转录因子VaERF99功能及其作用机理[博士论文]. 杨凌: 西北农林科技大学.
[5]	Choquer M, Fournier E, Kunz C, Levis C, Pradier J, Simon A, Viaud M. 2007. Botrytis cinerea virulence factors:new insights into a necrotrophic and polyphageous pathogen. FEMS Microbiology Letters,(277):1-10.
[6]	Coelho J, Almeida-Trapp M, Pimentel D, Soares F, Reis P, Rego C, Mithöfer A, Fortes A M. 2019. The study of hormonal metabolism of Trincadeira and Syrah cultivars indicates new roles of salicylic acid,jasmonates,ABA and IAA during grape ripening and upon infection with Botrytis cinerea. Plant Science,283:266-277.
[7]	Dean R, van Kan J A, Pretorius Z A, Hammond-Kosack K E, DiPietro A, Spanu P D. 2012. The Top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol,13:414-430.
[8]	Fan F, Hamada M S, Li N, Li G Q, Luo C X. 2017. Multiple fungicide resistance in Botrytis cinerea from greenhouse strawberries in Hubei Province,China. Plant Disease, 101 (4):601-606. doi: 10.1094/PDIS-09-16-1227-RE pmid: 30677353
[9]	Fang Xianping, He Yani, Xi Xiaojun, Zha Qian, Zhang Liqing, Jiang Aili. 2019. Multi-omics reveals the resistance mechanism of grape leaves in response to Botrytis cinerea. Journal of Zhejiang University(Agriculture & Life Sciences),45 (3):306-316. (in Chinese)
	方献平, 和雅妮, 奚晓军, 查倩, 张丽勍, 蒋爱丽. 2019. 多组学技术揭示葡萄叶片响应灰葡萄孢菌侵染的抗性机制. 浙江大学学报(农业与生命科学版), 45 (3):306-316.
[10]	Floryszak-Wieczorek J, Arasimowicz-Jelonek M. 2017. The multifunctional face of plant carbonic anhydrase. Plant Physiology and Biochemistry,112:362-368.
[11]	Frick U B, Schaller A. 2002. cDNA microarray analysis of fusicoccin-induced changes in gene expression in tomato plants. Planta, 216 (1):83-94. doi: 10.1007/s00425-002-0887-1 pmid: 12430017
[12]	Götz R, Gnann A, Zimmermann F K. 1999. Deletion of the carbonic anhydrase-like gene NCE103 of the yeast Saccharomyces cerevisiae causes an oxygen-sensitive growth defect. Yeast,15:855-864.
[13]	Gullner G, Komives T, Kiraly L, Schroder P. 2018. Glutathione S-transferase enzymes in plant-pathogen interactions. Front Plant Sci,9:1836.
[14]	Lee E S, Kang C H, Park J H, Lee S Y. 2017. Physiological significance of plant peroxiredoxins and the structure-related and multifunctional biochemistry of peroxiredoxin 1. Antioxidants & Redox Signaling, 28 (7):625-639.
[15]	Lei D, Lin Y, Luo M, Zhao B, Tang H, Zhou X, Yao W, Zhang Y, Wang Y, Li M, Chen Q, Luo Y, Wang X, Tang H, Zhang Y. 2022. Genome-wide investigation of G6PDH gene in strawberry:evolution and expression analysis during development and stress. International Journal of Molecular Sciences, 23 (9):4728.
[16]	Li R, Cheng Y. 2023. Recent advances in mechanisms underlying defense responses of horticultural crops to Botrytis cinerea. Horticulturae, 9 (11):1178.
[17]	Li L, Guo P, Jin H, Li T. 2016. Different Proteomics of Ca²⁺ on SA-induced resistance to Botrytis cinerea in tomato. Horticultural Plant Journal, 2 (3):154-162.
[18]	Li T, Zhang J, Tang J, Liu Z, Li Y, Chen J, Zou L. 2020. Combined use of Trichoderma atroviride CCTCCSBW0199 and brassinolide to control Botrytis cinerea infection in tomato. Plant Disease, 104 (5):1298-1304.
[19]	Meng X, Xu J, He Y, Yang K, Mordorski B, Liu Y, Zhang S. 2013. Phosphorylation of an ERF transcription factor by Arabidopsis MPK3/MPK 6 regulates plant defense gene induction and fungal resistance. The Plant Cell, 25 (3):1126-1142.
[20]	Mengiste T. 2012. Plant immunity to necrotrophs. Annual Review of Phytopathology, 50 (1):267-294.
[21]	Mosbach A, Edel D, Farmer A D, Widdison S, Barchietto T, Dietrich R A, Corran A, Scalliet G. 2017. Anilinopyrimidine resistance in Botrytis cinereais linked to mitochondrial function. Front Microbiol,8:2361.
[22]	Myresiotis C K, Karaoglanidis G S, Tzavella-Klonari K. 2007. Resistance of Botrytis cinerea isolates from vegetable crops to anilinopyrimidine, phenylpyrrole,hydroxyanilide,benzimidazole,and dicarboximide fungicides. Plant Dis, 91 (4):407-413. doi: 10.1094/PDIS-91-4-0407 pmid: 30781182
[23]	Petrasch S, Knapp S J, Van Kan J, Blanco-Ulate B. 2019. Grey mould of strawberry,a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea. Mol Plant Pathol, 20 (6):877-892.
[24]	Sarven M S, Hao Q, Deng J, Yang F, Wang G, Xiao Y, Xiao X. 2020. Biological control of tomato gray mold caused by Botrytis cinerea with the entomopathogenic fungus Metarhizium anisopliae. Pathogens, 9 (3):213.
[25]	Shah P, Powell A L, Orlando R, Bergmann C, Gutierrez-Sanchez G. 2012. Proteomic analysis of ripening tomato fruit infected by Botrytis cinerea. J Proteome Res, 11 (4):2178-2192.
[26]	Tola A J, Jaballi A, Germain H, Missihoun T D. 2020. Recent development on plant aldehyde dehydrogenase enzymes and their functions in plant development and stress signaling. Genes(Basel),12:51.
[27]	Tong Jianhua, Gicharu G K, Hu Xun, Fan Xiaojing, Zhuo Tao, Zou Huasong. 2019. NADH dehydrogenase subunit F is required for cell motility and pathogenicity in Ralstonia solanacearum. Acta Microbiologica Sinica, 59 (10):1960-1968. (in Chinese)
	童建华, Gicharu G K, 户勋, 范晓静, 卓涛, 邹华松. 2019. 茄科作物青枯菌NADH脱氢酶F亚基在细胞运动和致病性中的功能研究. 微生物学报, 59 (10):1960-1968.
[28]	Upadhyay R K, Mattoo A K. 2018. Genome-wide identification of tomato(Solanum lycopersicum L.) lipoxygenases coupled with expression profiles during plant development and in response to methyl-jasmonate and wounding. Journal of Plant Physiology,231:318-328.
[29]	Wan Ran. 2016. Researches on the mechanism for Chineses wild Vitis germplasm leaves against Botrytis cinerea[Ph. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	万然. 2016. 中国野生葡萄种质叶片抗灰霉病的机制研究[博士论文]: 杨凌: 西北农林科技大学.
[30]	Wang Mengnan. 2018. Cloning and functional analysisof a VaERF20 transcription factor in Chinese wild Vitis amurensis[Ph. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	王梦楠. 2018. 中国野生山葡萄转录因子VaERF20克隆与功能研究[博士论文]. 杨凌: 西北农林科技大学.
[31]	Wei X, Huang X, Yang W, Wang X, Guan T, Kang Z, Liu J. 2022. A chloroplast-localized glucose-6-phosphate dehydrogenase positively regulates stripe rust resistance in wheat. Int J Mol Sci,24:459.
[32]	Yan C, Yang N, Li R, Wang X, Xu Y, Zhang C, Wang X, Wang Y. 2023. Alfin-like transcription factor VqAL4 regulates a stilbene synthase to enhance powdery mildew resistance in grapevine. Molecular Plant Pathology, 24 (2):123-141.
[33]	Yan L, Zhai Q, Wei J, Li S, Wang B, Huang T, Du M, Sun J, Kang L, Li C B, Li C. 2013. Role of tomato lipoxygenase D in wound-induced jasmonate biosynthesis and plant immunity to insect herbivores. PLoS Genet, 9 (12):e1003964.
[34]	Yu Dingqun, Tang Haoru, Zhang Yong, Luo Ya, Liu Zejing. 2012. Research progress in glucose-6-phosphate dehydrogenase in higher plants. Chinese Journal of Biotechnology, 28 (7):800-812. (in Chinese) pmid: 23167192
	于定群, 汤浩茹, 张勇, 罗娅, 刘泽静. 2012. 高等植物葡萄糖-6-磷酸脱氢酶的研究进展. 生物工程学报, 28 (7):800-812. pmid: 23167192
[35]	Zhang J, Li Y, Du S, Deng Z, Liang Q, Song G, Wang H, Yan M, Wang X. 2023. Transcriptomic and proteomic analysis reveals (E)-2-hexenal modulates tomato resistance against Botrytis cinerea by regulating plant defense mechanism. Plant Mol Biol, 111 (6):505-522.
[36]	Zhu Y, Zhang X, Zhang Q, Chai S, Yin W, Gao M, Li Z, Wang X. 2022. The transcription factors VaERF16 and VaMYB306 interact to enhance resistance of grapevine to Botrytis cinerea infection. Mol Plant Pathol, 23 (10):1415-1432.
[37]	Zhu Yanxun. 2022. Screening of key ERF genes for grape resistance to Botrytis cinerea and the regulatory mechanism of VaERF16 against B. cinerea[Ph. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	朱彦勋. 2022. 葡萄抗灰霉病关键ERF基因筛选及VaERF16抗灰霉病调控机理[博士论文]. 杨凌: 西北农林科技大学.

蛋白 Protein	基因ID Gene ID	描述 Description	引物序列（5′-3′） Primer sequence
A0A438C3M1	PNC1_16	过氧化物酶Peroxidase	F：GTTTGTGCCCTGGGGTTGTTTC
A0A438C3M1	PNC1_16		R：ACTCGCCGTGGTTGAATCTCTTC
A0A438C992	GSTU10_1	谷胱甘肽转移酶Glutathione transferase	F：TGAGGTAGTTGGTGTGGTTGTCG
A0A438C992	GSTU10_1		R：GTCTCCTTCATCAGTGGGCAGTC
D7TAQ3	XM_002265469	脂氧合酶Lipoxygenase	F：TGAGGACAAGACTGTGGAAGGTG
D7TAQ3	XM_002265469		R：AGAAGACGACGACGACGACAAC
D7SL57	XM_002285430	乙醛脱氢酶Aldehyde dehydrogenase	F：TTCACACACGAGAAGGCGATCC
D7SL57	XM_002285430		R：TCAAGCCCAAGAGGACAAGCAG
A5AEM3	XM_003634025	二苯乙烯合酶Stilbene synthase 4	F：AACGACGACAGGTGAAGGATTGG
A5AEM3	XM_003634025		R：TGGAGCACAACGGTCTCAATGG
D7TU30	XM_010649825	碳酸酐酶Carbonic anhydrase	F：TGGCCTTCTCATCCTCCTCTCTC
D7TU30	XM_010649825		R：TGGGTCTGCTCAATTACGCTCAG
A5C501	XM_002265668	NADH脱氢酶NADH dehydrogenase [ubiquinone] iron-sulfur protein 4，mitochondrial	F：AGTCACTGCAACGGCTATGGAG R：CGATCTCGCCCGGTTTGATTTC
A0A438JBL2	POX2_1	脯氨酸脱氢酶Proline dehydrogenase	F：GCAGGATGTGGAGGAGGCTTG
A0A438JBL2	POX2_1		R：AGGTGGCGTTATCGGTGGTATG
A0A438CM14	POXN1_5	过氧化物酶Peroxidase	F：GAGGGCTGCGTGGATTGAATTTC
A0A438CM14	POXN1_5		R：TCCCAGTCTTCACCCCAATGTTG
A0A438IMB3	VvCHDp000857_1	葡萄糖-6-磷酸脱氢酶 Glucose-6-phosphate-dehydrogenase	F：TTCGTCTTTCTCGGCACCCTTC R：AATGGCGGCTGATGGTACTGAG

蛋白 Protein	基因ID Gene ID	描述 Description	引物序列（5′-3′） Primer sequence
A0A438C3M1	PNC1_16	过氧化物酶Peroxidase	F：GTTTGTGCCCTGGGGTTGTTTC
A0A438C3M1	PNC1_16		R：ACTCGCCGTGGTTGAATCTCTTC
A0A438C992	GSTU10_1	谷胱甘肽转移酶Glutathione transferase	F：TGAGGTAGTTGGTGTGGTTGTCG
A0A438C992	GSTU10_1		R：GTCTCCTTCATCAGTGGGCAGTC
D7TAQ3	XM_002265469	脂氧合酶Lipoxygenase	F：TGAGGACAAGACTGTGGAAGGTG
D7TAQ3	XM_002265469		R：AGAAGACGACGACGACGACAAC
D7SL57	XM_002285430	乙醛脱氢酶Aldehyde dehydrogenase	F：TTCACACACGAGAAGGCGATCC
D7SL57	XM_002285430		R：TCAAGCCCAAGAGGACAAGCAG
A5AEM3	XM_003634025	二苯乙烯合酶Stilbene synthase 4	F：AACGACGACAGGTGAAGGATTGG
A5AEM3	XM_003634025		R：TGGAGCACAACGGTCTCAATGG
D7TU30	XM_010649825	碳酸酐酶Carbonic anhydrase	F：TGGCCTTCTCATCCTCCTCTCTC
D7TU30	XM_010649825		R：TGGGTCTGCTCAATTACGCTCAG
A5C501	XM_002265668	NADH脱氢酶NADH dehydrogenase [ubiquinone] iron-sulfur protein 4，mitochondrial	F：AGTCACTGCAACGGCTATGGAG R：CGATCTCGCCCGGTTTGATTTC
A0A438JBL2	POX2_1	脯氨酸脱氢酶Proline dehydrogenase	F：GCAGGATGTGGAGGAGGCTTG
A0A438JBL2	POX2_1		R：AGGTGGCGTTATCGGTGGTATG
A0A438CM14	POXN1_5	过氧化物酶Peroxidase	F：GAGGGCTGCGTGGATTGAATTTC
A0A438CM14	POXN1_5		R：TCCCAGTCTTCACCCCAATGTTG
A0A438IMB3	VvCHDp000857_1	葡萄糖-6-磷酸脱氢酶 Glucose-6-phosphate-dehydrogenase	F：TTCGTCTTTCTCGGCACCCTTC R：AATGGCGGCTGATGGTACTGAG

品种 Cultivar	显著差异蛋白 Significant difference protein	上调 Up-regulation	下调 Down-regulation
黑比诺Pinot Noir	868	543	325
双优Shuangyou	1 946	969	977

品种 Cultivar	显著差异蛋白 Significant difference protein	上调 Up-regulation	下调 Down-regulation
黑比诺Pinot Noir	868	543	325
双优Shuangyou	1 946	969	977

品种 Cultivar	表达 Express	蛋白ID Protein ID	描述 Description	log₂FC
黑比诺 Pinot Noir	上调 Up-regulated	A0A438JBL2	脯氨酸脱氢酶Proline dehydrogenase	3.71
		A0A438CM14	过氧化物酶Peroxidase	3.66
		A0A438IMB3	葡萄糖-6-磷酸脱氢酶Glucose-6-phosphate-dehydrogenase	2.15
双优 Shuangyou	上调 Up-regulated	A0A438C3M1	过氧化物酶Peroxidase	3.56
		A0A438C992	谷胱甘肽转移酶Glutathione S-transferase	3.31
		D7TAQ3	脂氧合酶Lipoxygenase	2.11
		D7SL57	乙醛脱氢酶Aldehyde dehydrogenase	2.09
		A5AEM3	二苯乙烯合酶Stilbene synthase 4	1.65
	下调 Down-regulated	D7TU30	碳酸酐酶Carbonic anhydrase	-2.89
	下调 Down-regulated	A5C501	NADH脱氢酶NADH dehydrogenase[ubiquinone] iron-sulfur protein 4，mitochondrial	-2.72

Proteomics Studies on the Early Stages of Botrytis cinerea Infection in Grapevine Leaves

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