Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (11): 2251-2261.doi: 10.16420/j.issn.0513-353x.2021-0397

• Original article • Previous Articles     Next Articles

Reference Genes Selection and Validation in Begonia masoniana Leaves of Different Developmental Stages

YANG Ting1, XUE Zhenzhen1, LI Na2, LANG Xiaoan2, LI Lingfei2,*(), ZHONG Chunmei1,*()   

  1. 1Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Key Laboratory of Energy Plants Resource and Utilization,Ministry of Agriculture and Rural Affairs,Institute of Biomass Engineering,South China Agricultural University,Guangzhou 510642,China
    2Key Laboratory of Southern Subtropical Plant Diversity,Shenzhen Fairy Lake Botanical Garden,Chinese Academy of Sciences,Shenzhen,Guangdong 518005,China
  • Received:2021-05-17 Revised:2021-09-15 Published:2021-12-02
  • Contact: LI Lingfei,ZHONG Chunmei;


In order to research the suitable reference genes and the expression of the anthocyanin biosynthesis genes in the leaves of Begonia masoniana at different developmental stages,the expression stability of 11 genes(PP2A,EF1α,GADPH,CYP,elF4A,ACT2,18S,UBQ10,ACT7,PPR and HDH) was evaluated by real-time PCR,GeNorm,NormFinder and BestKeeper. The results showed that ACT7 and PP2A can be used as the suitable reference genes for gene expression analysis in B. masoniana. The expression levels of key genes involved in anthocyanin biosynthesis such as CHS,F3H,F3’H,FLS,DFR and UFGT were measured in leaves using the selected reference genes. The expression of the six anthocyanin pathway genes was higher in younger leaves and then declined during the later development stages,which was consistent with the change in the anthocyanin content in variegated leaves.

Key words: Begonia masoniana, quantitative real-time PCR, reference gene, anthocyanin biosynthesis genes, expression analysis

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