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Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (1): 73-87.doi: 10.16420/j.issn.0513-353x.2023-0632

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles     Next Articles

Mechanistic Studies on SlBON1-Regulated Plant Vegetative Growth in Tomato

LI Rui, WANG Wen, DU Minghui, LIU Genzhong, MA Fangfang*(), BAO Zhilong*()   

  1. State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Received:2024-08-29 Revised:2024-10-25 Online:2025-01-25 Published:2025-01-18
  • Contact: MA Fangfang, BAO Zhilong

Abstract:

BONZAI1(BON1)is a conserved calcium-dependent,plasma membrane-binding Copine protein,which is important in regulating plant growth and development. Tomato BON1 homolog(SlBON1)has 66.84% protein sequence similarity to Arabidopsis BON1. Crispr-Cas9 technology was utilized to generate two SlBON1 knockout mutants cr6 and cr8,which have 61-nucleotide missing and one-nucleotide insertion,respectively. The SlBON1 knockout mutants have the stunted growth of both aboveground and underground tissues,decreased endoreduplication in hypocotyls,reduced net photosynthetic rate,and increased starch accumulation in leaves compared to the wild type. Transcriptome analysis reveal that SlBON1 knockout mutants had a number of differentially expressed genes,in which hormone signaling pathway,MAPK signaling pathway and cell cycle regulation pathway are enriched. Taken together,the study suggest that SlBON1 plant hormone metabolism,modulates cell cycle and photosynthesis to promote tomato vegetative growth.

Key words: tomato, SlBON1, knock out, vegetative growth, RNA-seq analysis