Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1023-1030.doi: 10.16420/j.issn.0513-353x.2021-0732

• Research Papers • Previous Articles     Next Articles

PavMYB10.1 Promotes Anthocyanin Accumulation by Positively Regulating PavRiant in Sweet Cherry

LI Lixian1, WANG Shuo1, CHEN Ying1, WU Yingtao1, WANG Yaqian1, FANG Yue1, CHEN Xuesen1, TIAN Changping2, FENG Shouqian1,*()   

  1. 1State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
    2Yantai Academy of Agricultural Sciences,Yantai,Shandong 265599,China
  • Received:2022-01-04 Revised:2022-03-28 Online:2022-05-25 Published:2022-05-25
  • Contact: FENG Shouqian


To understand how GST regulates anthocyanin accumulation in sweet cherry,a phi-type GST gene PavRiant(XM_021968160)was cloned from the sweet cherry cultivar‘Tieton’. The effects of PavRiant on anthocyanin accumulation in sweet cherry were investigated using phylogenetic tree analysis,real-time reverse transcription-polymerase chain reaction,transient silencing assay,yeast one-hybrid(Y1H),electrophoretic mobility shift assay(EMSA),and luciferase reporter assay. Phylogenetic tree analysis showed that PavRiant has the highest similarity to the PpRiant protein sequence of peach. During sweet cherry fruit development,the changing trends of PavRiant expressions were consistent with anthocyanin contents and PavMYB10.1 expressions. Further,the transient silencing assay showed that PavRiant plays an essential role in regulating anthocyanin accumulation. Y1H and EMSA experiments showed that PavMYB10.1 binds to the MRE sequence of the PavRiant promoter. Finally,the luciferase assay showed that PavMYB10.1 positively regulates the activity of the PavRiant promoter. Therefore,PavMYB10.1 can promote anthocyanin accumulation by positively regulating PavRiant expression in sweet cherry.

Key words: sweet cherry, anthocyanin, PavMYB10.1, PavRiant, GST

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