Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 827-840.doi: 10.16420/j.issn.0513-353x.2020-1069

• Research Papers • Previous Articles     Next Articles

Identification and Expression Analysis of Genes Related to DNA Methylation in Chrysanthemum × morifolium and C. nankingense

WANG Ying, AI Penghui, LI Shuailei, KANG Dongru, LI Zhongai, WANG Zicheng**()   

  1. State Key Laboratory of Crop Stress Adaptation and Improvement,Laboratory of Plant Germplasm Resources and Genetic Engineering,Kaifeng Key Laboratory of Chrysanthemum Biology,School of Life Sciences,School of Agriculture,Henan University,Kaifeng,Henan 475004,China
  • Received:2021-07-16 Revised:2021-11-15 Online:2022-04-25 Published:2022-04-24
  • Contact: WANG Zicheng


In this study,based on the transcriptome data of the traditional chrysanthemum (Chrysanthemum × morifolium Ramat.)species‘Jinbeidahong’and the genome data of Chrysanthemum nankingense,the genes encoding DNA methylation-related enzyme were identified through bioinformatics from the‘Jinbeidahong’and C. nankingense,phylogenetic trees and gene expression were analyzed. Totally 13 DNA methyltransferase genes and 11 demethylase genes were identified from‘Jinbeidahong’,and 10 DNA methyltransferase genes and five demethylase genes were identified from C. nankingense. The evolutionary relationship demonstrated that C. lavandulifolium(Fisch. ex Trautv.)Ling et Shih were more closely associated with chrysanthemum‘Zijingling’‘Jinbeidahong’‘Hechengxinghuo’than C. nankingense and‘Quanxiangshuichang’. The transcripts levels of DNA methylation-related enzyme genes in multiple tissues and chrysanthemum varieties were quite different.

Key words: chrysanthemum, DNA methylation, DNA methyltransferase, DNA demethylase, gene identification, gene expression

CLC Number: