Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 816-826.doi: 10.16420/j.issn.0513-353x.2021-0007

• Research Papers • Previous Articles     Next Articles

Overexpression of Chrysanthemum indicum miR396a Gene in Arabidopsis Enhances Its Salt Tolerance

LIU Xiaowei1, XIA Bin1, LI Ziwei1, YANG Yujia1, CHEN Bin1, ZHOU Yunwei2,**(), HE Miao1,**()   

  1. 1. College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China
    2. College of Horticulture,Jilin Agricultural University,Changchun 130118,China
  • Received:2021-11-29 Revised:2022-03-07 Online:2022-04-25 Published:2022-04-24
  • Contact: ZHOU Yunwei,HE Miao;


cin-miR396a precursor sequence and its promoter were cloned from Chrysanthemum indicum;the precursor sequence alignment and evolutionary analysis were performed;the promoter was analyzed for cis-acting elements. The genetic transformation of the cin-miR396a precursor sequence to Arabidopsis thaliana was further carried out,and the salt tolerance of transgenic Arabidopsis thaliana was analyzed. The results showed that cin-MIR396a was 145 bp in length,contained an intact stem-loop structure,and was evolutionarily closest to A. thaliana ath-MIR396a. The promoter region has multiple response elements such as light signal,methyl jasmonate,gibberellin,salicylic acid,and abscisic acid,and it is hypothesized that cin-MIR396a is involved in response and regulation under a variety of adversity stresses. Overexpression of the cin-miR396a precursor in A. thaliana can improve seed germination under salt stress and salt tolerance at the adult seedling stage,and cin-miR396a plays a positive regulatory role in A. thaliana in response to salt stress.

Key words: Chrysanthemum indicum, miR396, Arabidopsis thaliana, transgenic, salt stress

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