Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 841-850.doi: 10.16420/j.issn.0513-353x.2021-0182

• Research Papers • Previous Articles     Next Articles

The Heterologous Expression of Oncidium OnGI Promotes Flowering in Arabidopsis thaliana

FANG Nengyan, FAN Ronghui, LUO Yuanhua, KONG Lan, LIN Rongyan, YE Xiuxian, LIN Bing, ZHONG Huaiqin*(), HUANG Minling*()   

  1. Fujian Engineering Research Center of Characteristic Floriculture,Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China
  • Received:2021-11-01 Revised:2022-03-22 Online:2022-04-25 Published:2022-04-24
  • Contact: ZHONG Huaiqin,HUANG Minling;


In this study,it was found that long-day condition could promote the flower bud differentiation of Oncidium under different photoperiod treatments. The OnGI gene of Oncidium was cloned,and its coding region was 3 483 bp long,encoding 1 161 amino acids. The results of multiple sequence alignment showed that it had the highest homology with Dendrobium nobile DnGI(86.57%). The phylogenetic tree analysis showed that it was classified into the same group with other orchids. Tissue expression analysis showed that the expression of OnGI was the highest in flowers,especially at the initial stage of bud formation,and in leaves at different growth stages,the expression of OnGI peaked at the stage of flower bud differentiation. Under different photoperiod conditions,the expression pattern of OnGI was different,similar to that of Arabidopsis thaliana. The results of subcellular localization showed that OnGI was localized in the nucleus. Overexpression of OnGI in Arabidopsis thaliana could lead to earlier flowering. Based on the above results,we speculate that there may be a similar photoperiod regulation mechanism of GI expression in Arabidopsis thaliana and to regulate plant flowering.

Key words: Oncidium, OnGI, photoperiod, expression, flowering

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