Abstract: The genetic relationship of 34 stone-used Ginkgo biloba L. cultivars were assessed by AFLP markers. A total of 1 337 bands were amplified with 8 pairs of primers，the percentage of polymorphic loci was 99.63%. The genetic similarity coefficient among 34 stone-used G. biloba L. cultivars ranged from 0.2915 to 0.6812 with an average of 0.4911，and all cultivars were divided into 4 groups at 0.50 of the similarity coefficient. The fingerprint of G. biloba L. cultivars were established based on AFLP results. Nei’s gene diversity index ranged from 0.1392 to 0.2014 for 4 types of stone-used G. biloba L. cultivars，with the total value of 0.2112. Shannon’s information index varied from 0.2138 to 0.3165，with the total value of 0.3417. The genetic differentiation coefficient for 4 types of stone-used G. biloba L. cultivars was 0.0871 and genetic flow was 5.4230. Eight cultivars were recognized relatively more distinctive among 34 stone-used G. biloba L. cultivars.

Key words: Ginkgo biloba, stone-used, AFLP, genetic diversity, genetic relationship