Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (7): 1282-1294.doi: 10.16420/j.issn.0513-353x.2021-0242

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Association Analysis Between Background Selection InDel Markers and Important Agronomic Traits in Inbred Lines of Chinese Cabbage

ZHAO Yujing1, LU Yin1, FENG Daling1, LUO Lei1, WU Fang2, YANG Rui1, CHEN Yueqi1, SUN Xiaoxue1, WANG Yanhua1, CHEN Xueping1, SHEN Shuxing1,**(), LUO Shuangxia1,**(), ZHAO Jianjun1,**()   

  1. 1State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei/Collaborative Innovation Center of Vegetable Industry in Hebei/College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001,China
    2Institute of Economic Crops,Hebei Academy of agriculture and Forestry Sciences,Shijiazhuang 050051,China
  • Received:2021-04-27 Revised:2021-05-21 Online:2021-07-25 Published:2021-08-10
  • Contact: SHEN Shuxing,LUO Shuangxia,ZHAO Jianjun;;


The InDel marker for genetic background selection is beneficial to the efficient selection of Chinese cabbage breeding parents. Taking 31 inbred lines of Chinese cabbage with different ecological types as materials,InDel genetic background and association of agronomic traits were analyzed in this study. The results showed that the coefficient of variation of 31 agronomic traits varied from 15.97% to 47.63%,with an average of 32.58%,indicating that the phenotypic diversity of tested population was rich. Finally,158 polymorphic primer pairs were selected from 177 InDel primer pairs. A total of 701 polymorphic alleles were detected in 31 accessions of Chinese cabbage,with an average of 4.418 alleles per primer pair,ranging from 1 to 11. The polymorphic information content(PIC)of the marker loci ranged from 0.612 to 0.963,with an average of 0.843,and the number of effective alleles(Ne)ranged from 1.656 to 3.985,averagely 2.640. The genetic similarity coefficient(GS)of tested materials of Chinese cabbage varied greatly from 0.575 to 0.910,indicating that the genetic background of the population was quite different. According to population structure analysis,tested materials were divided into three subgroups. Association analysis showed that 22 InDel molecular markers distributed in nine linkage groups related to 12 agronomic traits,including expansion spreading,whole plant weight,head leaf number,outer leaf number,leaf width,leaf length,leaf head height,leaf head width,leaf head weight,middle rib length,central column length and plant height.

Key words: Chinese cabbage, genetic diversity, InDel, association analysis, background selection

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