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ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (8): 1554-1562.

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Expression Analysis of Seven Dehydrin Genes in Loquat

XU Hong-xia1,2,LI Xiao-ying2,CHEN Jun-wei2,FENG Jian-jun3,and XU Chang-jie1,*   

  1. (1The State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China;2Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;3Department of Agricultural and Forestry of Ninghai County,Ninghai,Zhejiang 315600,China)
  • Received:2014-04-22 Online:2014-08-25 Published:2014-08-25

Abstract: In order to further reveal the possible functions of different dehydrin family members in loquat(Eriobotrya japonica Lindl.)(EjDHN),the expression patterns of seven EjDHNs were analyzed with various organs,fruit at different development stages,leaves from different seasons,leaves under drought stress and ABA treatment in cultivar‘Ninghaibai’. It was observed that EjDHN5 was highly expressed in all organs tested,EjDHN4 was mainly expressed in flower,while EjDHN2EjDHN3EjDHN6 and EjDHN7 were expressed especially high in stem. In fruit,all members,only except for EjDHN3,responded to seed development. The expression levels of EjDHN2 and EjDHN5 were relatively high in pulp and responsed to pulp development. In leaves during seasonal changes,the expression pattern of different EjDHN members was rather similar,with the highest transcript levels reached in winter,whereas the expression were also strong in early summer for EjDHN1EjDHN2EjDHN4 and EjDHN5. Under drought stress,the transcript levels of all seven genes increased in leaves,with EjDHN6 most greatly increased. Moreover,expression of all these genes responded differently,with EjDHN6 most sensitive,to ABA treatment.

Key words: loquat, dehydrin, gene expression, fruit developmental stage, seasonal changes, drought stress, ABA

CLC Number: