Abstract: Using transcriptom sequencing technology to analysis and detected different expressed gene among monoecious，andromonecious，gynoecious and hermaphrodite families from WI998（gynoecious）cross Topmark（andromonecious），a full length of 1 842 bp which locates in chromosome 12，named CmTs2，was obtained from bioinformatics methods. Sequence analysis indicated that the CmTs2 consisting an open reading frame（ORF）which from 119 to 832 nucleotides encoding 237 amino acids with molecular weight 25 358.8 D，instability index is 21.6 and aliphatic index is 99.11，grand average of hydropathicity is 0.078. Blast alignment analysis indicated highly homologous to TS2 gene especially for cucumber which has 96% homologous relationship，pathway analysis indicated CmTs2 gene located the upstream of gibberellin synthesis pathway. Real time-PCR analysis indicated that the expression level of CmTs2 was highest expressed in andromonecious but lowest in gynoecious in leaf and flower，which showed the CmTs2 gene might involve in the flower development female flower.

Key words: Cucumis melo, transcriptom sequence, sex expression, gene expression