Abstract: A CBF/DREB gene named FaCBF1 was cloned from strawberry（Fragaria × ananassa）through SON-PCR and RT-PCR and its GenBank accession number is FJ767754. The FaCBF1 gene from the cDNA contained an open reading frame of 636 bp，which encoded a polypeptide of 211 amino acids residues with a molecular mass of 23.4 kD and a pI of 6.53. The sequence homology comparison showed that the FaCBF1 exhibits a high homology of the deduced amino acids sequences with other Rosaceae CBF/DREB from GenBank. A phylogeny tree showed that the FaCBF1 had a relatively close evolutionary relationship with Rosa hybrida，Malus × domestica and Pyrus pyrifolia，and a distant relationship with Prunus plants. Semi-quantitative RT-PCR analysis showed that the expression of FaCBF1 was induced by low temperature，drought，and salt stress，but insensitivity to ABA treatment. The expression level in root was higher than leaf and stem at the same treatment time.

Key words: Fragaria × ananassa, FaCBF1, gene cloning, gene expression