Abstract: In this research，SCoT markers were applied to detect the genetic diversity among 24 Cymbidum samples of 14 species. Two hundred and fifty-nine DNA bands were generated by 27 primers including 224 polymorphic bands. The polymorphic percent is 86.49%. Strip size was from 100 bp to 2 000 bp，and most of the bands were concentrated between 500 bp and 1 300 bp. Dendrograms generated from coefficient of genetic distance between species on the basis of SCoT markers showed that the germplasms were divided into 5 clusters. Cluster A was composed of C. georingii，C. ensifolium，C. faberi，and C. tortosepalum；Cluster B included C. sinese and C. kanran；C. goeringii var. ongibracteatum was cluster C；C. lancifolium belonged to cluster D；Cluster E was composed of C. floribundum，C. wenshanense，C. hookerianum，C. maguanense，C. lowianum and C. iridioides. The research showed that part of the result was different from the traditional classification of Cymbidium.

Key words: Cymbidum；molecular marker, SCoT, genetic diversity