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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 325-335.doi: 10.16420/j.issn.0513-353x.2020-0262

• Research Notes • Previous Articles     Next Articles

Association Analysis of SCoT Markers and Leaf Phenotypic Traits in Cherry Germplasm

PENG Fangfang1, LONG Zhijian2, WEI Zhaoxin1, LI Xunlan1, LUO Youjin1, HAN Guohui1,*()   

  1. 1Fruit Research Institute,Chongqing Agriculture Science Academys,Chongqing 401329,China
    2College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China
  • Received:2020-10-15 Revised:2020-12-17 Online:2021-02-25 Published:2021-03-09
  • Contact: HAN Guohui E-mail:hghui2007@126.com

Abstract:

Based on the established SCoT analysis system,the suitable primers were screened,and then SCoT molecular markers,genetic diversity of leaf traits and their correlation were analyzed in 12 cherry varieties. The results showed that 46 marker primers being suitable for cherry genetic analysis were selected from 102 SCoT marker primers,with a screening rate of 45.1%. A total of 77 allele points were obtained from 18 SCoT primers,including 65 polymorphic loci,with a polymorphism rate of 84.4%. The variation coefficient of leaf traits of 12 varieties was 11.49%-36.93%,which was basically consistent with the results of SCoT marker clustering analysis. Pearson’s correlation analysis showed that a total of 54 marker sites were significantly or extremely significantly correlated with 12 leaf traits. Through the multiple linear stepwise regression analysis,eight specific marker sites related to leaf traits were obtained, which explained the change of 47.8%-100.0% of leaf traits. Sequence analysis of eight association marker sites showed that five markers could find homologous sequences in cherry genome;the similarity was more than 75%;and their chromosome positions could be identified. Among them,four markers could find homologous sequences with functional annotation in NCBI,and three markers could not find homologous sequences.

Key words: cherry, SCoT markers, leaf traits, association analysis

CLC Number: