Abstract: Secondary structure，transmembrane region，hydrophobicity/ hydrophilicity，subcellular localization and conserved structure domains of MdGLRs proteins were predicted and analyzed with bioinformatics methods，alignment of amino acid sequences and phylogenetic analysis. The expression of MdGLRs genes was analyzed in different vegetative organs with semi-quantitative RT-PCRs. The results showed that MdGLRs family contained 32 genes，which was further divided into 3 sub-families. Most MdGLRs protein contained more than 800 amino acids. They generally had 3 transmembrane regions. The secondary structures of MdGLRs proteins were mainly alpha helix，random coil and extended strand. The prediction of sub-cellular localization showed that MdGLRs proteins were mainly localized in endoplasmic reticulum and plasma membrane. Both MdGLRs and AtGLRs proteins contained conserved structure domains. Expression analysis showed that most genes expressed in roots，stems and leaves，with the highest expression level in leaves.

Key words: glutamate receptors, MdGLRs family genes, sequence analysis, bioinformatics, expression analysis