Genetic Analysis and SSR Markers of Acid Gene Ma in Apple

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (10): 1885-1892.

• Fruit Trees • Previous Articles Next Articles

Genetic Analysis and SSR Markers of Acid Gene Ma in Apple

WANG Lei-Cun, FAN Hong-Ke, GAO Hua, ZHAO Zheng-Yang

（1College of Horticulture，Northwest A & F University，Yangling，Shaanxi 712100，China；2Shaanxi Rural Science and Technology Development Center，Xi’an 710054，China）

Online:2012-10-25 Published:2012-10-25

Abstract

Abstract: Researches on heredity characters of acid contents and molecular markers of genes controlling acidness can provide supplementary means for apple breeding in quality.A DNA marker linked to the acid gene（Ma） in apple was explored based on the population of 216 progenies of‘Spur Fuji’×‘Pink Lady’by simple sequence repeat（SSR） technique combined with bulk segregation analysis（BSA）. One hundred and two primer pairs were screened，and two SSR markers（CH03d12₁₀₄+ CH03d12₁₁₈） closely linked to Ma gene was identified. The linkage distance of two markers locus is 3.24 and 2.31 cM，respectively. The SSR marker analysis showed that the fruit acid trait was governed by Ma₁and Ma₂，and Ma was completely dominant to ma.

Key words: apple, Ma gene, SSR marker, genetic analysis

CLC Number:

S 661.1

WANG Lei-Cun, FAN Hong-Ke, GAO Hua, ZHAO Zheng-Yang. Genetic Analysis and SSR Markers of Acid Gene Ma in Apple[J]. ACTA HORTICULTURAE SINICA, 2012, 39(10): 1885-1892.

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Genetic Analysis and SSR Markers of Acid Gene Ma in Apple

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