Abstract: In order to analyze glucosinolate（GS）composition and content in various organs and varieties of tuber mustard（Brassica juncea var. tumida Tsen et Lee），tissues of roots，swollen stems，leaves and floral buds of four varieties were sampled at floral bud emergence stage. A total of nine glucosinolates were detected in different varieties and various organs，including four aliphatic glucosinolates（2-Propenyl GS，3-Butenyl GS，1-Methylpropyl GS and 1-Methybutyl GS），four indole glucosinolates（4-Hydroxyindol-3-ylmethyl GS，Indol-3-ylmethyl GS，4-Methoxyindol-3-ylmethyl GS and 1-Methoxyindol-3-ylmethyl GS），and one aromatic glucosinolate（2-phenylethyl GS）.Total glucosinolate contents in roots，swollen stems，leaves and floral buds ranged from 13.632 to 20.155 μmol · g-1 DW，25.916 to 36.487 μmol · g-1 DW，63.715 to 96.259 μmol · g-1 DW and 89.764 to 129.763 μmol · g-1 DW，respectively. Floral buds contained the highest concentration，followed by leaves，swollen stem and roots. 2-phenylethyl GS and 2-Propenyl GS were the major glucosinolates in roots，accounting for 34.99%–61.87% and 13.54%–47.53% of the total root GSs，respectively. The major glucosinolate in swollen stems，leaves and floral buds was 2-propenyl GS，accounting for 81.38%–93.04%，85.45%–95.99% and 88.60%–95.18% of the total glucosinolates，respectively. Significant differences of total and individual glucosinolate levels were observed among different organs and varieties.

Key words: tuber mustard （Brassica juncea var. tumida Tsen et Lee）, variety, organ, glucosinolate, composition, content