Abstract: Diploid grape‘Muscat Hamburg’，‘Red Globe’，‘Moldova’and‘Otilia seedless’were crossed with tetraploid parents‘Kyoho’. The hybrid embryos were in vitro cultured and regenerated into plantlets. The regeneration rate of plantlets reaches to 30.6%. By using Flow Cytometry，thirty triploid cross progenies were obtained. The results showed that the percentages of triploid cross progenies was different when with the different cross combinations of parents. Recently 225 regenerated grape progenies were transplanted into field. 　　

Key words: grape, embryo culture, triploid grape breeding, identification of chromosome