ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (8): 1227-1232.

• 研究简报 • Previous Articles     Next Articles

Study on Genetic Diversity of Some Rosa Germplasm in Yunnan Based on SSR Markers

TANG Kai-xue1,2, QIU Xian-qin2, ZHANG Hao2, LI Shu-fa2, WANG Qi-gang2, JIAN Hong-ying2, YAN Bo2, and HUANG Xing-qi1,3*

  1. (1College of Life Sciences, Yunnan University, Kunming 650091,China;2 Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;3 Yunnan Key Laboratory of Agricultural Biotechnology, Kunming 650223, China)
  • Received:2008-04-02 Revised:2008-07-16 Online:2008-08-25 Published:2008-08-25
  • Contact: HUANG Xing-qi

Abstract: The genetic diversity of 42 Rosa germplasm including 13 accessions of wild species (varieties , forms) and 29 rose cultivars in Yunnan was studied by simple sequence repeat (SSR). Eighteen pairs of SSR primers were used in PCR amplification with genomic DNA as template. A total of 148 alleles were detected at 18 loci. The number of alleles per locus ranged from 6 to 14, with an average of 8.2. The genetic similar coefficient ranged from 0.282 to 0.892, which showed that the genetic diversity among 42 rose accessions in Yunnan was abundant based on SSR molecular markers. The 13 Rosa species could be divided into five groups obviously based on the UPGMA cluster at the similar coefficient 0.456,which is consensus of botanical morphological classification. All the species and cultivars were divided into five groups at the similar coefficient of 0.43 by UPGMA cluster. Meanwhile genetic relationships between 42 rose accessions were discussed preliminarily.

Key words: Rosa L., genetic diversity, genetic relationship, SSR

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