Cloning and Functional Analysis of a Novel Chymopapain Promoter from Carica papaya

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (7): 973-978.

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Cloning and Functional Analysis of a Novel Chymopapain Promoter from Carica papaya

YANG Ying-jun^1,2,ZHOU Peng^2*,LI Yan-mei¹, and SHEN Wen-tao²

(¹College of Forestry，Henan University of Science and Technology， Luoyang Henan 471003，China; ² National Key Biotechnology Laboratory for Tropical Crops Institute of Bioscience and Biotechnology，Chinese Academy of Tropical Agricultural Sciences，Haikou Hainan 571101，China）

Received:2008-01-02 Revised:2008-05-26 Online:2008-07-25 Published:2008-07-25
Contact: ZHOU Peng

Abstract

Abstract:

A partial gene sequence and 5' flanking sequence of chymopapain were isolated from the genomic DNA of Carica papaya via PCR technology. The results of alignment indicated that the isolated DNA sequence had 99% homology with that of the chymopapain gene in papaya. The core promoter regions and some upstream regulatory elements in this fragment were analyzed. Transcriptional start site（TSS）was T predicted by the software of PROMOTER PREDICTION and PLANT CARE TATA-box，CAAT-box，WUN，HSE regions and other cis-elements were found incooespanding promoter sequence regions with others. Compared with the data in GenBank，the results showed that a novel promoter was obtained，the GenBank accession number was AY803756. Binary vectors were then constructed，GUS expressions were both observed in papaya leaves transferred via particle bombardment and tobacco plantlet mediated by agrobacterium. GUS activities were detected only in latex.

Key words: Carica papaya, chymopapain, promoter, sequence analysis, gene expression, laticifer

CLC Number:

S 667.9

YANG Ying-jun;ZHOU Peng;LI Yan-mei;and SHEN Wen-tao. Cloning and Functional Analysis of a Novel Chymopapain Promoter from Carica papaya [J]. ACTA HORTICULTURAE SINICA, 2008, 35(7): 973-978.

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Cloning and Functional Analysis of a Novel Chymopapain Promoter from Carica papaya

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