Abstract:

In this paper, the feasibility of using Affymetrix Vitis vinifera gene expression microarray to detect the gene expression of Chinese wild grape was studied, and the gene expression 48 h after artificial inoculation with Uncinular necator was detected. The results showed that the microarray can detect the expression of 11 906 genes in susceptible material (S), while 11 839 in resistant individual (R) and in both R and S 11 839 genes. The detected genes accounted for ~71.3% of the total 16 602 probe groups. That means Affymetrix V. vinifera microarray can be efficiently used for gene expression analysis of Chinese wild grapes. Compared to the S plant, 1 920 up regulated and 1 760 down regulated genes were detected in R plant, accounting for 16.21% and 14.87% of total detected genes respectively, up and down regulated rate (signal log ratio) are between 1~7.

Key words: grapevine, gene microarray, disease resistance gene, gene expression