Transgene Expression in Pear (Pyrus communis L.) Driven by Phloem-specific Promoter AtSUC2

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (4): 487-492.

• 果树 • Previous Articles Next Articles

Transgene Expression in Pear (Pyrus communis L.) Driven by Phloem-specific Promoter AtSUC2

SUN Qing-rong^1* , SUN Hong-yan¹ , ZHAO Yan², HAMMOND Rosemarie², and DAVIS Robert E.²

(¹Shandong Institute of Pomology, Tai'an, Shandong 271000, China; ²Molecular Plant Pathology Laboratory, USDA-Agriculture Research Service, Beltsville, MD 20705, U.S.A.)

Received:2007-12-27 Revised:1900-01-01 Online:2008-04-25 Published:2008-04-25
Contact: SUN Qing-rong

Abstract

Abstract:

Leaves of 'Old Home' Pear (Pyrus communis L.) in vitro were selected as explants. GUS (β-glucuronidase) reporter gene (uidA) under the control of the Arabidopsis sucrose-H+ symporter gene (AtSUC2) promoter which is phloem-specific was introduced into 'Old Home' by Agrobacterium tumefaciens. Optimal conditions were established for efficient transformation from leaf explants of a pear cultivar, 'Old Home'. High transformation efficiency was achieved by an improved induction stage following initial Agrobacterium infection. In the induction stage, Agrobacterium cells and parent leaf explants were co-cultivated on a liquid induction medium, which yielded a five-fold increase of transformation frequency over conventional co-cultivation on a solid medium. The integration was checked by PCR and Southern hybridization. The expression of uidA gene was analyzed by GUS histochemical detection and Western blot analysis.

Key words: pear, promoter, phloem-specific expression, transgene

CLC Number:

S 661.2

SUN Qing-rong; SUN Hong-yan; ZHAO Yan; HAMMOND Rosemarie; and DAVIS Robert E. . Transgene Expression in Pear (Pyrus communis L.) Driven by Phloem-specific Promoter AtSUC2 [J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 487-492.

[1]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, and YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[2]	YUAN Xin, XU Yunhe, ZHANG Yupei, SHAN Nan, CHEN Chuying, WAN Chunpeng, KAI Wenbin, ZHAI Xiawan, CHEN Jinyin, GAN Zengyu. Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit [J]. Acta Horticulturae Sinica, 2023, 50(1): 53-64.
[3]	SONG Jiankun, YANG Yingjie, LI Dingli, MA Chunhui, WANG Caihong, and WANG Ran. A New Pear Cultivar‘Luxiu’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 3-4.
[4]	DONG Xingguang, CAO Yufen, ZHANG Ying, TIAN Luming, HUO Hongliang, QI Dan, XU Jiayu, LIU Chao, and WANG Lidong. A New Cold-resistant Crispy Pear Cultivar‘Yucuixiang’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 5-6.
[5]	OU Chunqing, JIANG Shuling, WANG Fei, MA Li, ZHANG Yanjie, and LIU Zhenjie. A New Early-ripening Pear Cultivar‘Xingli Mishui’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 7-8.
[6]	ZHANG Yanjie, WANG Fei, OU Chunqing, MA Li, JIANG Shuling, and LIU Zhenjie. A New Pear Cultivar‘Zhongli Yucui 3’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 9-10.
[7]	WANG Suke, LI Xiugen, YANG Jian, WANG Long, SU Yanli, ZHANG Xiangzhan, and XUE Huabai. A New Red Pear Cultivar‘Danxiahong’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 13-14.
[8]	WANG Fei, OU Chunqing, ZHANG Yanjie, MA Li, and JIANG Shuling. A New Late Ripening Pear Cultivar‘Huaqiu’with Long Storage Period [J]. Acta Horticulturae Sinica, 2022, 49(S1): 9-10.
[9]	SONG Jiankun, LI Dingli, YANG Yingjie, MA Chunhui, WANG Caihong, and WANG Ran. A New Pear Cultivar‘Qindaohong’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 11-12.
[10]	GUO Weizhen, ZHAO Jingxian, LI Ying. A New Mid-early Ripening Pear Cultivar‘Meiyu’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2051-2052.
[11]	LIU Jinming, GUO Caihua, YUAN Xing, KANG Chao, QUAN Shaowen, NIU Jianxin. Genome-wide Identification of Dof Family Genes and Expression Analysis Sepal Persistent and Abscission in Pear [J]. Acta Horticulturae Sinica, 2022, 49(8): 1637-1649.
[12]	TAO Xin, ZHU Rongxiang, GONG Xin, WU Lei, ZHANG Shaoling, ZHAO Jianrong, ZHANG Huping. Fructokinase Gene PpyFRK5 Plays an Important Role in Sucrose Accumulation of Pear Fruit [J]. Acta Horticulturae Sinica, 2022, 49(7): 1429-1440.
[13]	LIANG Qin, ZHANG Yanhui, KANG Kaiquan, LIU Jinhang, LI Liang, FENG Yu, WANG Chao, YANG Chao, LI Yongyu. Molecular Evolution of MiR168 Family and Their Expression Profiling During Dormancy of Pyrus pyrifolia [J]. Acta Horticulturae Sinica, 2022, 49(5): 958-972.
[14]	XIANG Miaolian, WU Fan, LI Shucheng, MA Qiaoli, WANG Yinbao, XIAO Liuhua, CHEN Jinyin, CHEN Ming. Exogenous Melatonin Regulates Reactive Oxygen Metabolism to Induce Resistance of Postharvest Pear Fruit to Black Spot [J]. Acta Horticulturae Sinica, 2022, 49(5): 1102-1110.
[15]	WANG Dan, WANG Mi, LIU Jun, ZHOU Xiaohui, LIU Songyu, YANG Yan, ZHUANG Yong. Cloning of U6 Promoters and Establishment of CRISPR/Cas9 Mediated Gene Editing System in Eggplant [J]. Acta Horticulturae Sinica, 2022, 49(4): 791-800.

Transgene Expression in Pear (Pyrus communis L.) Driven by Phloem-specific Promoter AtSUC2

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments