Exogenous Melatonin Regulates Reactive Oxygen Metabolism to Induce Resistance of Postharvest Pear Fruit to Black Spot

doi:10.16420/j.issn.0513-353x.2021-0159

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1102-1110.doi: 10.16420/j.issn.0513-353x.2021-0159

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Exogenous Melatonin Regulates Reactive Oxygen Metabolism to Induce Resistance of Postharvest Pear Fruit to Black Spot

XIANG Miaolian¹, WU Fan¹, LI Shucheng¹, MA Qiaoli¹, WANG Yinbao¹, XIAO Liuhua¹, CHEN Jinyin¹^,², CHEN Ming¹^,^*()

¹Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province,Jiangxi Key Laboratory for Postharvest Technology and Non-destructive Testing of Fruits & Vegetables,College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China
²Pingxiang University,Pingxiang,Jiangxi 337055,China

Received:2022-01-12 Revised:2022-02-28 Online:2022-05-25 Published:2022-05-25
Contact: CHEN Ming E-mail:mingchen@jxau.edu.cn

Abstract

Abstract:

‘Cuiguan’pears were used as materials to explore the effect of exogenous melatonin（MT）on the induced resistance to postharvest pear black spot caused by Alternaria alternata. To this end,the resistance to A. alternata and black spot were detected in MT treated pear fruits. The results showed that MT treatment（0.025-0.300 mmol · L^-1）had no significant inhibition on mycelial growth and spore germination rate of A. alternata. However,it could remarkably reduce black spot of pear fruit and 0.100 mmol · L^-1 MT treatment had the highest induced resistance effect of 45.04%. In comparison with the control,the activities of CAT,SOD,POD,APX and PPO,as well as the content of total phenolics,were obviously increased in 0.100 mmol · L^-1 MT treated fruits and the accumulation of MDA was just effectively reduced on the contrary. The above results indicated that exogenous melatonin inducing pear fruit resistance against A. alternata infection might be related to the regulation of reactive oxygen species metabolism.

Key words: pear, fruit, black spot, Alternaria alternata, melatonin, induced resistance, reactive oxygen metabolism

CLC Number:

S661.2

XIANG Miaolian, WU Fan, LI Shucheng, MA Qiaoli, WANG Yinbao, XIAO Liuhua, CHEN Jinyin, CHEN Ming. Exogenous Melatonin Regulates Reactive Oxygen Metabolism to Induce Resistance of Postharvest Pear Fruit to Black Spot[J]. Acta Horticulturae Sinica, 2022, 49(5): 1102-1110.

Figures/Tables 5

References 33

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MT/（mmol · L^-1）	孢子萌发率/% Spore germination rate	菌落直径/cm Diameter of colony
0（对照Control）	98.67 ± 1.25 a	4.83 ± 0.31 a
0.025	98.00 ± 1.41 a	4.67 ± 0.25 a
0.050	97.33 ± 1.25 a	4.68 ± 0.10 a
0.100	98.00 ± 0.82 a	4.57 ± 0.12 a
0.200	97.67 ± 1.25 a	4.87 ± 0.05 a
0.300	98.33 ± 1.24 a	4.53 ± 0.12 a

MT/（mmol · L^-1）	孢子萌发率/% Spore germination rate	菌落直径/cm Diameter of colony
0（对照Control）	98.67 ± 1.25 a	4.83 ± 0.31 a
0.025	98.00 ± 1.41 a	4.67 ± 0.25 a
0.050	97.33 ± 1.25 a	4.68 ± 0.10 a
0.100	98.00 ± 0.82 a	4.57 ± 0.12 a
0.200	97.67 ± 1.25 a	4.87 ± 0.05 a
0.300	98.33 ± 1.24 a	4.53 ± 0.12 a

MT/（mmol · L^-1）	病斑直径/mm Lesion diameter	诱导效应/% Induction effect
0（对照Control）	23.96 ± 2.76 a	—
0.025	15.63 ± 1.69 bc	34.78 ± 3.54 bc
0.050	14.29 ± 0.83 cd	40.35 ± 1.73 b
0.100	13.17 ± 1.34 d	45.04 ± 2.80 a
0.200	16.38 ± 2.60 b	31.65 ± 5.41 c
0.300	16.58 ± 1.89 b	30.78 ± 3.94 c

MT/（mmol · L^-1）	病斑直径/mm Lesion diameter	诱导效应/% Induction effect
0（对照Control）	23.96 ± 2.76 a	—
0.025	15.63 ± 1.69 bc	34.78 ± 3.54 bc
0.050	14.29 ± 0.83 cd	40.35 ± 1.73 b
0.100	13.17 ± 1.34 d	45.04 ± 2.80 a
0.200	16.38 ± 2.60 b	31.65 ± 5.41 c
0.300	16.58 ± 1.89 b	30.78 ± 3.94 c

时间/h Time	病斑直径/mm Lesion diameter	诱导效应/% Induction effect
0（对照Control）	20.38 ± 2.45 a	—
24	15.74 ± 0.64 b	22.77 ± 3.16 d
36	12.53 ± 0.98 d	38.51 ± 4.36 b
48	11.21 ± 0.86 e	44.96 ± 4.22 a
60	12.00 ± 0.69 de	41.10 ± 3.37 ab
72	13.63 ± 1.54 c	33.13 ± 3.57 c

Exogenous Melatonin Regulates Reactive Oxygen Metabolism to Induce Resistance of Postharvest Pear Fruit to Black Spot

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